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Application of Aleuritopteris argentea Fee extract in preparation of drugs treating leukemia

A technology of extracts of Herba Tongjing, which is applied in the field of medicine and can solve problems such as the reports of leukemia drugs of Herba Tongjing or its extracts.

Inactive Publication Date: 2014-02-19
白小英
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The above-mentioned documents and patents, etc., have not yet seen the report of Tongjingcao or Tongjingcao extract being used to prepare medicines for treating leukemia

Method used

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  • Application of Aleuritopteris argentea Fee extract in preparation of drugs treating leukemia
  • Application of Aleuritopteris argentea Fee extract in preparation of drugs treating leukemia
  • Application of Aleuritopteris argentea Fee extract in preparation of drugs treating leukemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Preparation of Tongjingcao Extract and Monomer Compounds

[0037] (1) 20kg of Herba Trichonia, using 60% ethanol as a solvent, soaking and extracting at 50°C, extracting 4 times, each extraction time is 4 hours, and the amount of solvent used for each time is 12 times the weight of Herba Trichonia; filter , combine the extracts, recover ethanol, concentrate, and dry to obtain Tongjingcao extract A;

[0038] (2) Add 12 times the amount of water to dissolve the Tongjingcao extract A obtained in step (1), and filter to obtain medicinal solution A and precipitate A. The precipitate A was separated and purified by high-speed countercurrent chromatography (HSCCC) using cyclohexane-ethyl acetate-n-butanol-water (3:2:3:1) as the solvent system, the solvent was recovered, concentrated and dried, That is tongjingcao extract B;

[0039] (3) Pass the drug solution A obtained in step (2) through an X-5 macroporous adsorption resin column, elute with water, and then elut...

Embodiment 2

[0043] Example 2: Preparation of Tongjingcao Extract and Monomer Compounds

[0044] (1) 10kg of Herba Trichonia, using 30% ethanol as a solvent, warm-soaked and extracted at 70°C, the number of extractions was 2 times, each extraction time was 2 hours, and the amount of solvent used each time was 8 times the weight of Herba Trichonia; Filtrate, combine the extracts, recover ethanol, concentrate, and dry to obtain Tongjingcao extract A;

[0045] (2) Add 6 times the amount of water to dissolve the Tongjingcao extract A obtained in step (1), and filter to obtain medicinal solution A and precipitate A. The precipitate A was separated and purified by high-speed countercurrent chromatography (HSCCC) using cyclohexane-ethyl acetate-n-butanol-water (3:2:3:1) as the solvent system, the solvent was recovered, concentrated and dried, That is tongjingcao extract B;

[0046] (3) Pass the drug solution A obtained in step (2) through the NKA macroporous adsorption resin column, elute with ...

Embodiment 3

[0050] Example 3: Preparation of Tongjingcao Extract and Monomer Compounds

[0051] (1) 5 kg of Herba Trichonia, using 80% ethanol as a solvent, warm-soaked and extracted at 30°C, the number of extractions was 5 times, each extraction time was 6 hours, and the amount of solvent used each time was 15 times the weight of Herba Trichonia; Filtrate, combine the extracts, recover ethanol, concentrate, and dry to obtain Tongjingcao extract A;

[0052] (2) Add 18 times the amount of water to dissolve the Tongjingcao extract A obtained in step (1), and filter to obtain medicinal solution A and precipitate A. The precipitate A was separated and purified by high-speed countercurrent chromatography (HSCCC) using cyclohexane-ethyl acetate-n-butanol-water (3:2:3:1) as the solvent system, the solvent was recovered, concentrated and dried, That is tongjingcao extract B;

[0053] (3) Pass the drug solution A obtained in step (2) through an ADS-8 macroporous adsorption resin column, elute wi...

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Abstract

Belonging to the medicine field, the invention relates to application of an Aleuritopteris argentea Fee extract in preparation of drugs treating leukemia by enhancing NCK, IL-2, IL-6 and IFN-gamma activity. The Aleuritopteris argentea Fee extract is prepared by: taking Aleuritopteris argentea Fee as the raw material, conducting extraction with 30%-80% ethanol, and carrying out high-speed counter-current chromatography, macroporous adsorption resin and other chromatography purification.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to the application of a traditional Chinese medicine extract in the preparation of a medicine for treating leukemia, in particular to the application of the extract of Tongjingcao in the preparation of a medicine for treating leukemia. Background technique [0002] Leukemia is a malignant disease that originates from the hematopoietic system and is characterized by the production and accumulation of a large number of immature and abnormal white blood cells in the bone marrow or other hematopoietic tissues. From the perspective of form, most leukemia cells are immature and abnormally morphological white blood cells; in terms of function, leukemia cells have the same characteristics as tumor cells: leukemia cells are tumorous proliferation, which cannot be controlled, and often leads to the inhibition and failure of bone marrow hematopoietic function. It can cause anemia, neutropenia and thrombo...

Claims

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Application Information

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IPC IPC(8): A61K36/11A61P35/02
Inventor 孔倩倩
Owner 白小英
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