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Application of madder polysaccharide in preparation of health-protection food with effects of preventing and treating neurodegenerative diseases

A neurodegenerative, health food technology, applied in the functions, applications, food preparation and other directions of food ingredients, can solve the problems such as the preparation process needs to be improved, the development and utilization are few, and the research and application have not yet been reported, so as to delay the prevention and treatment of neurodegeneration. Sexual diseases, the effect of solving the extensive extraction process and the simple and perfect preparation process

Active Publication Date: 2014-02-12
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current preparation process of polysaccharides in traditional Chinese medicine is relatively extensive, and there are problems such as low polysaccharide content, many impurities such as small molecules and proteins, negative charge and uneven molecular weight distribution, and the development of its active functions is mostly focused on immune regulation, anti-tumor and In terms of anti-oxidation, the research on neuroprotection has not been in-depth, and the corresponding development and utilization are very few
For example, studies have shown that Rubia polysaccharide has multiple functions such as anti-oxidation and anti-radiation, but its research and application in the field of neurodegenerative diseases has not been reported, and its preparation process still needs to be improved

Method used

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  • Application of madder polysaccharide in preparation of health-protection food with effects of preventing and treating neurodegenerative diseases
  • Application of madder polysaccharide in preparation of health-protection food with effects of preventing and treating neurodegenerative diseases
  • Application of madder polysaccharide in preparation of health-protection food with effects of preventing and treating neurodegenerative diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030]A Rubia acid polysaccharide provided in this example is prepared by the following method: crush 500 g of Rubia rhizome, add ethanol according to the ratio of solid to liquid 1:10 (g / mL), heat, stir and reflux at 70°C for 3 hours, and filter under reduced pressure The solvent is removed and the medicinal materials are recovered. Add water according to the solid-liquid ratio of 1:20 (g / mL), heat and stir for extraction at 90°C for 3h. Concentrate under reduced pressure and centrifuge to obtain a clarified extract, add papain at a concentration of 2.0 g / mL, and heat and stir at 40° C. and pH 6.0 for 2 h. After the enzymolysis, add 4 times (enzymolysis solution volume) volume of ethanol into the enzymolysis solution, mix well and let it settle overnight at 4°C, collect polysaccharides by centrifugation, and obtain crude polysaccharides by vacuum freeze-drying. The crude polysaccharide was dissolved in water, passed through 732 type cation exchange resin, and eluted with wat...

Embodiment 2

[0037] A rubia acid polysaccharide provided in this example is prepared by the following method: crush 500 g of rubia rhizome, add ethanol according to the ratio of solid to liquid at 1:5 (g / mL), heat, stir and reflux at 80°C for 3 hours, and filter under reduced pressure The solvent is removed and the medicinal materials are recovered. Add water according to the solid-liquid ratio of 1:10 (g / mL), heat and stir for extraction at 100°C for 3 hours. Concentrate under reduced pressure and centrifuge to obtain a clarified extract, add papain at a concentration of 1.0 g / mL, and heat and stir at 50°C and pH 6.0 for 3 hours. After the enzymatic hydrolysis, add 4 times the volume of ethanol into the enzymatic hydrolysis solution, mix well and let it settle overnight at 4°C. The polysaccharide is collected by centrifugation, and the crude polysaccharide is obtained by vacuum freeze-drying. The crude polysaccharide was dissolved in water and passed through 732-type cation exchange resi...

Embodiment 3

[0044] A rubia acid polysaccharide provided in this example is prepared by the following method: crush 500 g of rubia rhizome, add ethanol according to the ratio of solid to liquid 1:20 (g / mL), heat and stir at 70°C for 2 hours, and filter under reduced pressure The solvent is removed and the medicinal materials are recovered. Add water according to the solid-liquid ratio of 1:10 (g / mL), heat and stir for extraction at 100°C for 3h. Concentrate under reduced pressure and centrifuge to obtain a clear extract, add pepsin at a concentration of 1.0 g / mL, and heat and stir at 37°C and pH 4.0 for 3 hours. After the enzymatic hydrolysis, 5 times the volume of ethanol was added to the enzymatic hydrolysis solution, mixed evenly and left to settle at 4°C overnight, the polysaccharide was collected by centrifugation, and the crude polysaccharide was obtained by vacuum freeze-drying. The crude polysaccharide was dissolved in water and passed through CM Sephadex C-25 cation-exchange dext...

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Abstract

The invention discloses application of a madder polysaccharide in the preparation of a health-protection food with effects of preventing and treating neurodegenerative diseases. The madder polysaccharide can be a commercially available product or a product prepared by an applicant and is preferably a madder acidic polysaccharide. The invention also discloses a preparation method of the madder acidic polysaccharide. The madder acidic polysaccharide prepared by using the method disclosed by the invention has the characteristics of high polysaccharides content, low impurity content and high uniformity. Furthermore, the preparation method is simple and high in efficiency and is suitable for industrial production. Experiments on an in-vitro aggregation model, mammalian cells and a caenorhabditis elegans disease model show that the madder polysaccharide can effectively suppress the abnormal aggregation of polyglutamine and beta-amyloid proteins and the neurotoxicity of the polyglutamine and the beta-amyloid proteins and also obviously reduce the reactive oxide species level of the disease model, so that the madder polysaccharide has a good preventing and treating effect on the neurodegenerative diseases.

Description

technical field [0001] The invention belongs to the technical field of health food / health food, and specifically relates to the application of Rubia polysaccharide in preparing health food with the effect of preventing and treating neurodegenerative diseases. technical background [0002] Neurodegenerative diseases are a class of progressive diseases characterized by the gradual decline and death of specific nerve cells and tissues, including Alzheimer's disease, Huntington's disease and Parkinson's disease. With the intensification of the aging trend of the global population, neurodegenerative diseases have become major diseases that seriously endanger human life, health and quality of life, and have attracted great attention from all over the world. Although drugs that relieve symptoms of patients have been marketed, these drugs Lack of attention to the onset and development of pre-disease. Therefore, it is undoubtedly of great significance to people's livelihood to activ...

Claims

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Application Information

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IPC IPC(8): A23L1/09A23L33/125
CPCA23L29/30A23V2002/00C08B37/006A23V2200/30
Inventor 马忠华马方励李海峰胡明华徐晓飞
Owner INFINITUS (CHINA) CO LTD
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