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Method for extracting and identifying aesculin, fraxin, fraxetin and resveratrol from ledum palustre l.var.angustum e.busch

A technology of aescin and resveratrol, which is applied in the field of extraction and identification of aescin, the extraction and identification of anti-oxidative active ingredients in Ledum lenticus, auretidin, aurettin and resveratrol, and can solve the cumbersome operation process, Antioxidant active ingredient analysis takes a long time, unfavorable for the extraction and identification of trace antioxidant ingredients, etc., to achieve the effect of increasing the diffusion rate, reducing the extraction time, and reducing the viscosity

Active Publication Date: 2014-02-05
SHENZHEN XINGYIN PHARML
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The traditional extraction and identification of antioxidant active ingredients not only takes a long time to analyze, but also has a cumbersome operation process, which is not conducive to the extraction and identification of trace antioxidant ingredients.

Method used

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  • Method for extracting and identifying aesculin, fraxin, fraxetin and resveratrol from ledum palustre l.var.angustum e.busch

Examples

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Embodiment 1

[0019] Embodiment 1 A method for extracting and identifying aescin, aescin, aurettin and resveratrol from Ledum saponica, comprising the following steps:

[0020] (1) Raw material selection and pretreatment: Take the twigs of Ledum spp. from Daxinganling as raw materials, dry them in the shade at room temperature, and make pretreatment samples with a length of 0.5-1.0 cm;

[0021] (2) Crude extract by pressurized solution extraction technology: Take 1.5 grams of the pretreated sample and mix it with double-weight sea sand, fill it in an accelerated solvent extraction tube, put it into a closed extraction box protected from light, and extract it at an extraction temperature of 115 ℃, under the conditions of extraction pressure 1500psi, use 65% of the volume fraction of the accelerated solvent extraction tube volume fraction of 65% methanol to extract statically for 26 minutes, collect the solvent, and use 35% of the volume fraction of the accelerated solvent extraction tube volu...

Embodiment 2

[0024] Embodiment 2 A method for extracting and identifying aescin, aurettin, aurettin and resveratrol from Ledum saponica, comprising the following steps:

[0025] (1) Raw material selection and pretreatment: Take the twigs of Ledum spp. from Daxinganling as raw materials, dry them in the shade at room temperature, and make pretreatment samples with a length of 0.5-1.0 cm;

[0026] (2) Crude extract by pressurized solution extraction technology: Take 1.1 g of the pretreated sample and mix it with double-weight sea sand, fill it in an accelerated solvent extraction tube, put it into a closed extraction box protected from light, and extract it at an extraction temperature of 115 ℃, under the conditions of extraction pressure 1500psi, use 60% of the accelerated solvent extraction tube volume fraction of 65% methanol to extract statically for 22 minutes, then collect the solvent, and use 30% of the volume fraction of the accelerated solvent extraction tube volume fraction to 65% met...

Embodiment 3

[0029] Embodiment 3 A method for extracting and identifying aescin, aescin, aurettin and resveratrol from Ledum saponifolium, comprising the following steps:

[0030] (1) Raw material selection and pretreatment: Take the twigs of Ledum spp. from Daxinganling as raw materials, dry them in the shade at room temperature, and make pretreatment samples with a length of 0.5-1.0 cm;

[0031] (2) Pressurized solution extraction technique to extract crude extract: Take 2 grams of pretreated sample and mix it with double-weight sea sand, fill it in an accelerated solvent extraction tube, put it into a closed and dark extraction box, and extract it at an extraction temperature of 115 ℃, under the conditions of extraction pressure 1500psi, use 70% of the volume fraction of the accelerated solvent extraction tube volume fraction of 65% methanol to extract statically for 29 minutes, then collect the solvent, and use 39% of the volume fraction of the volume fraction of the accelerated solvent...

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Abstract

The invention relates to a method for extracting and identifying aesculin, fraxin, fraxetin and resveratrol from ledum palustre l.var.angustum e.busch. The method comprises the following steps: selecting and preprocessing raw materials; adding methanol with the volume percent being 65% and statically extracting a crude extract through using a pressure liquid extract technology at the temperature of 115 DEG C for 20-30 minutes; identifying antioxidant components in an on-line manner through using an on-line HPLC-ABTS<+> process; performing reservation for 7.41 minutes so as to obtain a peak, namely the aesculin, performing the reservation for 11.71 minutes to obtain a peak, namely the fraxin, performing the reservation for 15.22 minutes to obtain a peak, namely the fraxetin and performing the reservation for 17.43 minutes to obtain a peak, namely the resveratrol. According to the method, the steps of extracting and identifying the antioxidant active components are simplified, the four antioxidant active components, namely the aesculin, the fraxin, the fraxetin and the resveratrol can be separated and identified at the same time through a one-time extract process, and the efficiency is remarkably improved.

Description

technical field [0001] The invention relates to a method for extracting and identifying antioxidative active ingredients in Ledum edulis, in particular to a method for extracting and identifying aescin, auretrin, aghetin and resveratrol from Ledum edulis, and belongs to the field of biotechnology. Background technique [0002] Ledum palustre (Ledum palustre L) is one of the main natural plants distributed in the Daxingan Mountains and Changbai Mountains in my country. It is a small shrub of the Rhododendron family Rhododendron. and Ledum palustre L.var.dilatatum Wahl are very rich in resources. Duxiang is widely used in traditional Chinese medicine. Its functions include: detoxification, detoxification, antibacterial, anticancer and antidiabetic, etc. It is often used to treat rheumatism, joint disease, cough, cold and other diseases. Modern studies have shown that Ledum plants are rich in volatile oils, terpenes, flavonoids and other biologically active compounds. Zuo Wenj...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 裴世春徐玖亮高建伟甄玉萍
Owner SHENZHEN XINGYIN PHARML
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