Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them

Abstract

The invention provides polypeptides having any cellulolytic activity, e.g., a cellulase activity, a endoglucanase, a cellobiohydrolase, a beta-glucosidase, a xylanase, a mannanse, a beta-xylosidase, an arabinofiiranosidase, and / or an oligomerase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having any cellulolytic activity, e.g., a cellulase activity, e.g., endoglucanase, cellobiohydrolase, beta-glucosidase, xylanase, mannanse, beta-xylosidase, arabinofuranosidase, and / or oligomerase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. In one aspect, the invention provides polypeptides having an oligomerase activity, e.g., enzymes that convert recalcitrant soluble oligomers to fermentable sugars in the saccharification of biomass. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural, food and feed processing and industrial contexts. The invention also provides compositions or products of manufacture comprising mixtures of enzymes comprising at least one enzyme of this invention.

Description

[0001] This application is a divisional application, the filing date of the original application is December 8, 2006, the application number is 200680054166.5 (PCT / US2006 / 046919), and the title of the invention is "cellulolytic enzymes, their encoding nucleic acids, and methods for preparing and using them method". [0002] governmental support [0003] This invention was made with US Government support under DOE Contract Nos. 1435-04-03-CA-70224, 1435-04-04-CA-70224, and DE-FC36-03GO13146 awarded by the Department of Energy. The US Government has certain rights in this invention. [0004] CD Submission [0005] The contents of the following CD-ROM submissions are hereby incorporated by reference in their entirety: Sequence Listing in Computer Readable Form (CRF) on CD-ROM (File Name: 564462014241, Date Recorded: December 6, 2006, Size: 1,843,200 bytes); One of three CD-ROM copies (copy 1) (filename: 564462014241, date of record: December 6, 2006, size: 1,843,200 bytes); one ...

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Problems solved by technology

However, due to the high production cost of ethanol, current economic conditions do not support its widespread use

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