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Keratin degrading bacteria NJK4

A technology of keratin degrading bacteria and strains, applied in the directions of bacteria, microorganisms, animal feed, etc., can solve problems such as shortage of feed protein resources, and achieve the effect of promoting sustainable development, protecting the environment, and having broad application prospects.

Inactive Publication Date: 2014-02-05
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a high-efficiency keratin-degrading bacterial strain for the development of new protein feed for the serious shortage of feed protein resources in production practice

Method used

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  • Keratin degrading bacteria NJK4

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: Isolation of Keratin Degrading Strains

[0015] Collect 2 g of samples from the soil environment where feathers have accumulated for a long time, add 10 mL of sterile saline, shake for 1 min, let stand for 5 min, absorb the supernatant to inoculate the nutrient broth medium, and culture at 37 ° C for 48 h.

[0016] Streak inoculation of the above-mentioned cultured strains on a feather meal medium plate, and culture at 37° C. for 24 hours. The formula of the feather meal culture medium is that 1L of distilled water contains: 5.0g sodium chloride, 20.0g feather meal, and 20.0g agar powder. Heat to dissolve, adjust pH to 7.5, and autoclave at 121°C for 15 minutes.

[0017] Purify the bacteria that produce larger hydrolysis circles in the above medium. A bacterium with a strong ability to hydrolyze keratin was obtained and named: NJK4. The bacterial strain NJK4 is classified and named as: Bacillus pumilus, and was deposited in the General Microorganism Center...

Embodiment 2

[0018] Embodiment 2: the expansion culture of above-mentioned bacterial strain NJK4

[0019] The formula of the seed medium is that 1L of distilled water contains: 10.0g peptone, 3.0g beef extract, 2.0g yeast extract, and 5.0g sodium chloride. Heat to dissolve, adjust pH to 7.5, and autoclave at 121°C for 15 minutes.

[0020] The bacterial strain NJK4 obtained above was inoculated into a 250mL Erlenmeyer flask containing 50mL of seed medium, and cultivated on a shaker at a speed of 120r / min for 24h at a temperature of 37°C to obtain a NJK4 liquid culture. The number of cells was 8×1013CFU / mL.

Embodiment 3

[0021] Embodiment 3: Utilize strain NJK4 to ferment and degrade feather keratin in feather fermentation medium

[0022] Take 1 mL of the NJK4 liquid culture obtained in Example 2 and inoculate it into a 500 mL Erlenmeyer flask containing 100 mL of feather fermentation medium, and culture it on a shaker at a speed of 180 r / min for 3 days at a temperature of 37° C. The formula of the feather fermentation medium is, 1L distilled water contains: 0.05g calcium chloride, 0.15g magnesium sulfate heptahydrate, 0.15g sodium chloride, 0.54g dipotassium hydrogen phosphate, 0.09g potassium dihydrogen phosphate, 20g feathers, adjusted pH7.5, autoclave at 121°C for 15 minutes.

[0023] The feathers in the culture medium increased with time, first the feather twigs gradually fell off, and then the feather stalks gradually broke, and the feathers were completely degraded after 3 days; the culture medium gradually became turbid, and the color gradually deepened, and finally became a uniform da...

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Abstract

The invention relates to a keratin degrading bacteria NJK4, belongs to the field of biological high technology. The bacterial strain NJK4 is classified and named as bacillus pumilus, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.3980. The NJK liquid culture is obtained by inoculating the bacterial strain in a seed medium and culturing for 24 hours in 120r / min at 37 DEG C. The bacterial strain is capable of degrading feather keratin in a feather fermentation medium, the degrading rate of the feather keratin is 85.76%, and the in vitro digestibility coefficient of the fermented feather powder is 94.73%. The keratin degrading bacteria NJK4 can be applied to the development and utilization of waste feather resources in poultry slaughtering, the waste feather is translated into a high-class protein feed source, the waste materials are recycled and the environment is protected, the sustainable development is promoted, and the keratin degrading bacteria NJK4 has a wide application prospect.

Description

technical field [0001] The invention provides a method for expanding the cultivation of keratin-degrading bacteria NJK4 and its application. Background technique [0002] The protein needed by livestock and poultry mainly comes from feed. my country’s protein feed resources are very scarce, especially in the serious shortage of high-quality protein feed such as soybean meal and fish meal, which greatly restricts the development of my country’s animal husbandry. People urgently need to speed up the development of new feed Technology research and development. Among many protein feed materials, the crude protein content of feather meal is as high as 80-85%, while the crude protein content of high-quality fish meal is only 72%. Therefore, the development of feather meal as protein feed has broad application prospects. As a by-product of the livestock and poultry slaughtering and processing industry, my country's waste feather resources are extremely rich, and the waste of a larg...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/10C12R1/07
Inventor 姚大伟杨德吉许家荣聂康康许俊才
Owner NANJING AGRICULTURAL UNIVERSITY
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