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Rapid propagation method of Paris polyphylla plant

A fast and high-quality technology, applied in the fields of botanical equipment and methods, horticultural methods, plant regeneration, etc.

Inactive Publication Date: 2014-02-05
CHENGDU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no report on further obtaining Huazhonglou tissue culture seedlings on the basis of Huazhonglou callus induction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Selection of explants and induction of callus: Select the rhizomes of Huazhonglou plants that grow healthy and free from diseases and insect pests, wash the soil with running water first, and then absorb the water on the surface, then cut off the adventitious roots on the rhizomes, and then in the Cut two stem nodes with buds at the front end of the rhizome on the ultra-clean workbench, first sterilize them with 0.1% mercuric chloride for 6 minutes, then rinse with sterile water for 3 to 4 times, then cut off the buds on them, and finally the rhizomes Stem nodes cut into 0.6cm 3 Insert MS+6-BA0.5mg·L after size -1 +NAA0.5mg·L -1 +2,4-D1.0mg·L -1 In the callus induction medium, first place it in a refrigerator at 4°C for 8 days at low temperature, and then transfer it to culture at a culture temperature of 12°C, 4 hours of light per day and a light intensity of 800 lx. After 55 days of culture Statistics show that the callus induction rate is 34.18%;

[0021] (2)...

Embodiment 2

[0026] (1) Selection of explants and induction of callus: Select the rhizomes of Huazhonglou plants that grow healthy and free from diseases and insect pests, wash the soil with running water first, and then absorb the water on the surface, then cut off the adventitious roots on the rhizomes, and then in the Cut the first stem node with the bud body at the front end of the rhizome on the ultra-clean workbench, first sterilize it with 0.1% mercury chloride for 9 minutes, then rinse it with sterile water for 5 times, cut off the bud body on the rhizome, and finally the rhizome node cut into 1.0cm 3 Insert MS+6-BA 1.0mg·L after size -1 +NAA1.5mg·L -1 +2,4-D1.5mg·L -1 In the callus induction medium, first place it in a refrigerator at 6°C for low temperature treatment for 15 days, then transfer it to culture at a culture temperature of 22°C, light for 6 hours a day, and a light intensity of 1000 lx. After 55 days of culture, Statistics show that the callus induction rate is 40....

Embodiment 3

[0032] (1) Selection of explants and induction of callus: Select the rhizomes of Huazhonglou plants that grow healthy and free from diseases and insect pests, wash the soil with running water first, and then absorb the water on the surface, then cut off the adventitious roots on the rhizomes, and then in the Cut the three stem nodes with buds at the front end of the rhizome on the ultra-clean workbench, first sterilize them with 0.15% mercury chloride for 10 minutes, then rinse them with sterile water for 5-6 times, then cut off the buds on them, and finally the rhizomes Stem nodes cut into 1.5cm 3 Insert MS+6-BA 2.0mg·L after size -1 +NAA2.0mg·L -1 +2,4-D3.0mg·L -1 In the callus induction medium, first place it in a refrigerator at 10°C for low temperature treatment for 18 days, and then transfer it to culture at a culture temperature of 25°C, 12 hours of light per day and a light intensity of 1200lx for culture. After 55 days of culture Statistics show that the callus ind...

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PUM

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Abstract

The invention discloses a rapid propagation method of a Paris polyphylla plant. The method comprises the steps of inducing calluses, differentiating adventitious buds, and carrying out rooting culture, seedling hardening and transplanting, wherein a culture medium for inducing and rapidly propagating the adventitious buds comprises MS, 1.0-4.0 mg.L<-1> of 6-BA, 0.1-1.0 mg.L<-1> of IAA, 0.1-1.0 mg.L<-1> of KT and 200-500 mg.L<-1> of ceftriaxone sodium, and the rooting culture medium comprises 1 / 2 MS, 0.1-1.0 mg.L<-1> of IBA, 0.1-0.5 mg.L<-1> of NAA and 0.1%-1.0% of activated carbon. According to the rapid propagation method, methods for inducing and rapidly propagating the adventitious buds and rooting, hardening and transplanting rootless seedlings are further presented based on the callus induction of the Paris polyphylla for the first time, a novel way is provided for the culturing of fine varieties of the Paris polyphylla and the mass and rapid propagation of tissue culture seedlings, and the batch production of the Paris polyphylla plant can be realized under the conditions of short time and low cost.

Description

technical field [0001] The invention relates to a method for quickly obtaining tissue culture seedlings of huazhonglou, in particular to a method for rapid propagation of huazhonglou plants. Background technique [0002] Chinese Pharmacopoeia stipulates that Paris polyphylla var. yunnanensis is the rhizome of Paris polyphylla var. yunnanensis and Paris polyphylla var. chinensis. The former is mainly produced in Yunnan, and the latter is mainly produced in Sichuan. The earliest record in "Shen Nong's Materia Medica" is Huachonglou. Zhonglou has the effects of clearing heat and detoxifying, reducing inflammation and relieving pain, promoting blood circulation and removing stasis, cooling liver and relieving convulsions. [0003] In recent years, the demand has increased greatly because of its discovery of antiviral and antitumor effects. Since the 1980s, Chonglou has become one of the most representative and globally influential medicinal plant resources in western medicinal...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王跃华王金鹏张珏陈燕刘银花宋超段茂华任鹏飞杨霞
Owner CHENGDU UNIV
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