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Application of CD226 extracellular domain protein in inhibition of tumor cell proliferation

A technology of tumor cell proliferation and CD226, which is applied in the preparation of proteins. The CD226 extracellular segment protein inhibits the proliferation of tumor cells, which can solve the problem of insignificant killing effect and achieve the effect of avoiding side effects.

Active Publication Date: 2015-05-27
IMMUNOPHARMACEUTIC INST OF HEFEI RUIDA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this killing effect is not obvious in tumor patients

Method used

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  • Application of CD226 extracellular domain protein in inhibition of tumor cell proliferation
  • Application of CD226 extracellular domain protein in inhibition of tumor cell proliferation
  • Application of CD226 extracellular domain protein in inhibition of tumor cell proliferation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Embodiment 1, the synthesis of the protein of the present invention and its functional detection

[0061] 1. Protein expression and purification

[0062] 1. Design of expression vector

[0063] 1) Select the fragment sequence of the protein

[0064] The three-dimensional structure of CD226 has not yet been analyzed. Using the Smart tool of the Expasy website for structure prediction, it is obtained that there may be two immunoglobulin-like domains outside the CD226 molecule, and its transmembrane region may be from amino acid residue 243 to amino acid residue 250. between. Afterwards, using Expasy’s secondary structure prediction tool Psipred, it was predicted that after the 249th amino acid residue, there is an alpha helix structure, which is presumed to be the beginning of the transmembrane region. Therefore, we chose to intercept the extracellular structure at the 248th residue. part.

[0065] 2) Vector construction and tag selection

[0066] Using the cDNA of h...

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PUM

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Abstract

The invention discloses an effect of inhibiting tumor of CD226 extracellular domain protein. The CD226 extracellular domain protein is a protein consisting of an amino acid sequence shown by SEQ ID NO:1. The protein disclosed by the invention can be combined with CD112 and CD155, can be used for effectively closing binding sites between CD226 and target cells and inhibiting the cellular poison activity of NK cells, can be used as an inhibitor of the NK cells for treating autoimmune diseases on the one hand, and can generate an inhibitory action on proliferation of tumor cells after being combined with CD112 or CD155 molecules on the surfaces of the tumor cells on the other hand. Therefore, the protein can be used for inhibiting the proliferation of tumors in a tumor patient with a low immunologic function. Moreover, because the protein is a human body natural protein, an immunologic reaction of an organism is rarely caused. The invention relates to application of the CD226 extracellular domain protein in preparation of medicaments for inhibiting tumor cell proliferation.

Description

technical field [0001] The invention mainly belongs to the field of tumor immunology, and mainly relates to the preparation and application of a protein involved in inhibiting tumor cell growth. Specifically, the present invention relates to the use of CD226 extracellular segment protein to inhibit the proliferation of tumor cells. Background technique [0002] Natural killer cells (Natural Killer, NK cells) play an extremely important role in innate immunity, mainly including killing tumor cells, killing virus-infected cells, killing intracellular parasites and killing fungi. NK cells mainly function directly in two ways; one is to produce inhibitory effects on target cells by secreting cytokines. The second is to activate NK cells to kill target cells through the recognition between NK cell surface receptors and ligands. [0003] NK cells function mainly through various activating receptors and inhibitory receptors on the surface of NK cells. CD226 molecule is an activa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/17A61P35/00
Inventor 田志刚侯胜科孙汭郑晓东魏海明
Owner IMMUNOPHARMACEUTIC INST OF HEFEI RUIDA CO LTD
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