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Application of germacrone in preparation of liver cancer treatment medicines

A technology for the treatment of liver cancer and gemmazone, applied in the field of medicine, can solve problems such as no effective therapeutic drugs, inconclusive anti-cancer effects, and inactivation of tumor suppressors

Inactive Publication Date: 2014-01-15
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The anti-cancer potential of the volatile oil of zedoary has attracted more and more attention and confirmation, but the specific chemical components and the mechanism of action in the volatile oil of zedoary are still in the research stage, especially zemaone, curcumol, curededione and other zedoary The anti-cancer effects of the three main components in volatile oils have been inconclusive
Chinese Patent (Application No. 02125900.3) discloses that Gemacone is used to fight tumor cells. Chinese Patent (Application No. 201010604066.3) discloses that Gemacone is used alone to treat cervical cancer, but it is not found that Gemacone is used alone to treat liver cancer. literature
[0004] The pathogenic factors of liver cancer have not been studied clearly so far. The current research believes that its predisposing factors include physical, chemical and other factors that lead to hepatocyte proliferation, especially the uncontrolled cycle regulation and the inactivation or expression of tumor suppressors. diseases for which there is currently no effective treatment

Method used

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  • Application of germacrone in preparation of liver cancer treatment medicines
  • Application of germacrone in preparation of liver cancer treatment medicines
  • Application of germacrone in preparation of liver cancer treatment medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1 Gemaserone compares the proliferation inhibitory effect of HepG2 cells

[0020] 1. Experimental materials

[0021] 1.1 Cell lines

[0022] The human liver cancer cell line HepG2 was purchased from China Center for Type Culture Collection (CCTCC).

[0023] 1.2 Drugs and reagents

[0024] Gemmasone: purchased from Chengdu Master Biotechnology Co., Ltd.

[0025] DMSO: Product of Sigma, USA

[0026] DMEM and fetal bovine serum: American Gibco products

[0027] Penicillin-streptomycin solution: Anteje Biotechnology Co., Ltd.

[0028] MTT: Product of Sigma Company in the United States

[0029] Trypsin: Beyontian Institute of Biotechnology

[0030] Microplate reader Sunrise: TECAN company

[0031] Ultra-clean test bench SZX type: Shanghai Pudong Yuexin Instrument Factory

[0032] CO2 incubator HH·CP type: Shanghai Boxun Industrial Co., Ltd.

[0033] Micropipette P2.5, etc.: Dalong Medical Equipment Co., Ltd.

[0034] 2. Experimental method:

[0035] 2....

Embodiment 2

[0039] Example 2 Mechanism of Gemaserone Inhibiting HepG2 Cell Proliferation

[0040] 1. Experimental materials

[0041] 1.1 cell line (same as Example 1)

[0042] 1.2 Drugs and reagents

[0043] Gemmasone: purchased from Chengdu Master Biotechnology Co., Ltd.

[0044] DMSO: purchased from Sigma, USA

[0045] DMEM and fetal bovine serum: purchased from Gibco, USA

[0046] Western and IP cell lysates: purchased from Beyontien Institute of Biotechnology

[0047] PMSF: purchased from Biyuntian Biotechnology Research Institute

[0048] Hoechst 33258 staining solution: purchased from Biyuntian Institute of Biotechnology

[0049] MTT: Product of Sigma Company in the United States

[0050] Sodium dodecyl sulfate (SDS): Wuhan Dingguo Biotechnology Co., Ltd.

[0051] Glycine: Wuhan Dingguo Biotechnology Co., Ltd.

[0052] Nacl: Sinopharm Chemical Reagent Co., Ltd.

[0053] Methanol: Wuhan Dingguo Biotechnology Co., Ltd.

[0054] Trishydroxymethylaminomethane (Tris): Wuhan Ding...

Embodiment 3

[0131] The cytotoxicity of embodiment 3 gemaerone to human normal liver cells

[0132] 1. Experimental materials

[0133] 1.1 Cell lines

[0134] Human normal liver cell line (L02) was purchased from China Center for Type Culture Collection (CCTCC).

[0135] 1.2 Drugs and reagents: Same as Example 1.

[0136] 2 Experimental method: same as embodiment 1.

[0137] See the experimental results Figure 8 , From the results in the figure, we can see that gemmacone has no obvious inhibitory effect on the growth of normal human liver cell L02, which shows that gemmacone is an anti-hepatoma drug that is safe for normal cells.

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Abstract

The invention provides an application of germacrone as a liver cancer treatment medicine. Results of experiments about the cytotoxicity of germacrone to HepG2 cells show that IC50 is about 160mum; and results of experiments about the detection of the influences of germacrone to liver cancer HepG2 cell cycle and apoptosis by using a flow cytometer show that germacrone can induce HepG2 cell G2 / M cycle arrest and promote the cell apoptosis. Results of the detection of the cycle arrest and the apoptosis related protein expression situation by processing HepG2 cells through different concentrations of germacrone show that germacrone induces the HepG2 cell G2 / M cycle arrest by reducing the cyclinB1 and CDK1 proteins and promotes the HepG2 cell apoptosis through p53 regulated mitochondrial apoptosis. Results of the detection of the ROS output of the germacrone processed HepG2 cells show that germacrone can promote the rise of the ROS output and cause the oxidative damages of the cells.

Description

technical field [0001] The invention belongs to the field of medicine and relates to medicines for treating liver cancer, in particular to gemmaconone (molecular formula: C 15 h 22 O) Application in the preparation of drugs for the treatment of liver cancer. Background technique [0002] The key and foundation of the modernization of traditional Chinese medicine is the identification of its active ingredients and the study of the mechanism of action of the active ingredients. Therefore, it is of great significance to in-depth research on the active ingredients of traditional Chinese medicine, which will benefit the process of modernization of Chinese medicine and accelerate the globalization of Chinese medicine. Footsteps are more conducive to the protection of human health. [0003] The anti-cancer potential of the volatile oil of zedoary has attracted more and more attention and confirmation, but the specific chemical components and the mechanism of action in the volatil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/122A61P1/16A61P35/00
Inventor 何光源杨广笑柳昀熠方斌王维陈明洁
Owner HUAZHONG UNIV OF SCI & TECH
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