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Method of acquiring microscopic image with super field depth

A super-depth-of-field microscopy and image acquisition technology, applied in the field of microscopic vision, which can solve the problems affecting the portability of the microscopic imaging system

Inactive Publication Date: 2014-01-08
BEIHANG UNIV
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Problems solved by technology

At present, the main method used is to use the electric lifting table to drive the object under observation to move in the depth direction of the microscope to shoot the focused images at different depths of the object. Therefore, it is limited by the accuracy and stability of the electric lifting table, which also affects the microscopic imaging. system portability

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  • Method of acquiring microscopic image with super field depth
  • Method of acquiring microscopic image with super field depth
  • Method of acquiring microscopic image with super field depth

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Embodiment Construction

[0015] Embodiments according to the present invention will be described below with reference to the drawings. In the drawings, like reference numerals denote like elements throughout.

[0016] The optical path of the microscope imaging system according to the embodiment of the present invention is described with reference to the accompanying drawings.

[0017] figure 1 The flow chart of the method of the embodiment of the present invention is shown. By continuously changing the driving voltage of the liquid lens, we can clearly image the object under observation at various depths, and take a series of partially focused images. Then image fusion is performed on a series of locally focused images taken, and the fused images contain information in various depth ranges of the observed object, realizing the acquisition of ultra-depth-of-field microscopic images.

[0018] figure 2 The optical path diagram of the microscope system according to the embodiment of the present invent...

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Abstract

The invention provides a method of acquiring a microscopic image with super field depth. The method comprises the steps: adding a liquid lens with focal length controlled by voltage in a digital microscope light path, performing a series of focusing on the continuous depth range of an observed object by utilizing the liquid lens as a variable focal length element, shooting a series of continuous local focusing pictures, and fusing a super-field-depth clear image including focus information of all heights by utilizing an image fusion processing means. The position where the liquid lens is optimized so as to maintain the amplification factor unchanged in the focus variation process. According to the method, the problems that the traditional optical microscope is excessively small in field depth and objects in a certain depth range cannot be clearly imaged can be effectively solved.

Description

technical field [0001] The invention relates to the field of microscopic vision in the photoelectric detection technology, and more specifically relates to a method for acquiring ultra-depth-of-field microscopic images. Background technique [0002] The depth of field determines the range in which the visual inspection system can form a clear image in the direction of the optical axis. The larger the depth of field, the greater the range of depth that can be seen in the direction of the optical axis of the visual inspection system. Therefore, extending the depth of field is an urgent need for current visual inspection systems. [0003] Reducing the numerical aperture of the microscopic imaging system can effectively expand the depth of field, but due to the reduction of the aperture, the amount of light passing through is reduced and the resulting image contains greater noise. At the same time, reducing the aperture will also lead to a decrease in the resolution of the micro...

Claims

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Application Information

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IPC IPC(8): G02B21/36G02B21/24G02B26/02
Inventor 屈玉福杨海娟毛建森马诗洁
Owner BEIHANG UNIV
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