Preservation method for vitamin C mixed bacteria
A technology of vitamin and mixed bacteria, which is applied in the field of preservation of vitamin C mixed bacteria, can solve the problems that the stability cannot be effectively controlled and guaranteed, the proportion of bacteria and bacteria is out of balance, and the hidden dangers of product quality can be prevented, so as to prevent bacterial contamination or Seed solution quality degradation, fermentation process stability, and product quality assurance effects
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Embodiment 1
[0022] Example 1: Preparation and preservation of original bacterial strain cryopreservation tubes
[0023] 1 Take out 1ml of the seed liquid of the original bacterial strain that has been screened by shaking flasks and has excellent production performance and put it into 9ml sterile water with a concentration of 10 -1 , then from 10 -1 Inhale 1ml, put in another 9ml sterile water with a concentration of 10 -2 , and so on, diluted to 10 -8 , then from 10 -8 Aspirate 0.1ml into the separation plate, then spread it evenly with a triangular grill, and culture it in a constant temperature room at 29.5±1°C for 60-72 hours.
[0024] 2. Preparation of inclined plane: From the well-grown separation plate, pick well-grown, translucent, evenly distributed, and free of miscellaneous bacteria, and put them into sterile water to make the water of the small bacteria milky white. Use an inoculation loop or an inoculation stick to inoculate to On the inclined surface of the eggplant-shape...
Embodiment 2
[0027] Example 2: Preparation and preservation of cryopreservation tubes for bacterial strains used in production
[0028] 1 Take 0.5ml of the original bacterial strain cryopreservation tube, inoculate it into the seed medium (20ml), and cultivate it on a shaker at 29.5±1°C (rotating speed: 200 rpm) for 24 hours.
[0029] 2 Separate the cultured seed solution on the plate, spread it evenly, and cultivate it at 29.5±1°C for 2-3 days.
[0030] 3. From the cultured separation plate, select well-grown, translucent, evenly distributed, and free of miscellaneous bacteria, and put them into sterile water to make the water milky white, and inoculate them into a 250ml eggplant-shaped bottle with an inoculation loop or an inoculation stick. On the slope, wrap the mouth of the bottle with two layers of gauze, put it in a constant temperature room at 29.5±1°C for 22 to 24 hours, and observe that there are no bacteria growing, then transfer it to the inoculation room again. For moderately...
Embodiment 3
[0032] Embodiment 3: for the preparation of production seed solution
[0033] 1. Activation of the first generation: Take a cryopreservation tube for the strains used for production, and use a 1ml straw to transfer all the frozen liquid into a triangular flask containing 20ml of seed medium at 29.5±1°C, cultivate for 20-24 hours, and become the first generation seed liquid .
[0034] 2. Activate the second generation: absorb 5ml of the first generation seed solution and inoculate it into another seed medium (20ml) Erlenmeyer flask, at 29.5±1°C, shake and cultivate for 20±4 hours, which is the second generation seed solution.
[0035] 3. Activation of the third generation: inoculate 5ml of the second-generation seed liquid into another Erlenmeyer flask of seed medium (20ml), culture with shaking at 29.5±1°C for 20±4 hours. At this time, when it is detected that the acid production is above 5 mg / ml and there is no miscellaneous bacteria, it can be used for production.
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