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Ips cell having differentiation propensity for corneal epithelium

A technology of corneal epithelial cells and corneal epithelium, which is applied in the field of corneal epithelial differentiation-oriented iPS cells, can solve the problems of differentiation and induction of corneal epithelium, and achieve high safety effects

Inactive Publication Date: 2014-01-01
OSAKA UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current situation is that there is still no report on the induction of corneal epithelium from human ES cells and human iPS cells.

Method used

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  • Ips cell having differentiation propensity for corneal epithelium
  • Ips cell having differentiation propensity for corneal epithelium
  • Ips cell having differentiation propensity for corneal epithelium

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Experimental program
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Embodiment

[0128] Hereinafter, the present invention will be described in detail based on examples and the like, but the present invention is not limited thereto.

[0129] (I) Production of iPS cells derived from ocular epidermal cells

[0130] Human-derived corneal endothelial cells, surrounding junctional tissues, etc. are removed from the input sclerocorneal sheet for research, and only the corneal and conjunctival epithelial layer of the ocular surface is isolated by dispersion processing. The resulting epithelial cells were made into single cells by trypsin treatment, and cultured in a 1:1 mixed medium of NIH / 3T3 cell culture supernatant and fresh KCM medium.

[0131] Next, lentiviral vectors each carrying human Oct3 / 4, Sox2, Klf4, and c-Myc (Four Yamanaka factors) were infected in the proliferated ocular surface-derived epithelial cells. After 2-4 days after infection, the cells were re-inoculated into an iPS cell culture system (on MEF feeder cells in iPS medium), and cultured ...

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Abstract

The main purpose of the present invention is to provide a method for inducing the differentiation into a corneal epithelial stem cell and / or a corneal epithelial cell, which can be utilized for the production of a corneal epithelial cell sheet that has higher safety from the viewpoint of the possibility of occurrence of vascularization and the like and that can be produced readily. A method for inducing the differentiation of a pluripotent stem cell into a corneal epithelial stem cell and / or a corneal epithelial cell comprises a step of culturing the pluripotent stem cell in the presence of a stromal cell or an amnion-derived factor.

Description

technical field [0001] The present invention mainly relates to a method for inducing differentiation of pluripotent stem cells into corneal epithelial stem cells and / or corneal epithelial cells, a method for producing corneal epithelial stem cells and / or corneal epithelial cells, a corneal epithelial cell sheet derived from the corneal epithelial stem cells, and Corneal epithelial stem cells and / or induced pluripotent stem cells (iPS cells) used for differentiation induction of corneal epithelial cells. Background technique [0002] Maintaining good visual function is extremely important for QOL maintenance. Therefore, there is a high societal need to develop radical treatments for eye diseases in which visual function is impaired, especially for refractory eye diseases. As a group of refractory eye diseases, diseases such as Stevens-Johnson syndrome, ophthalmic pemphigoid, and alkali corrosion, which are corneal epithelial stem cell defects, are known. In corneal epitheli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10C12N5/071C12N5/0735
CPCC12N2501/603C12N2506/09C12N2501/602C12N2510/00C12N2501/606C12N2506/45C12N2502/1394C12N5/0602C12N5/0696C12N2501/604C12N5/0623C12N5/0621
Inventor 林龙平西田幸二
Owner OSAKA UNIV
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