Composition comprising banyan tree, lotus, and clover serum fractions (aging)

一种三叶草、组合物的技术，应用在哺乳动物皮肤的外观领域，能够解决减少、降低屏障能力、恢复能力降低等问题

Active Publication Date: 2014-01-01
NOURYON CHEM INT BV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Additionally, the barrier suffers from an age-related increase in free radical permeability and a decrease in the lipid content of the intercellular matrix, reducing the barrier's ability to disperse toxins from deeper layers
Barrier resilience to environmental damage is also greatly reduced with age

Method used

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  • Composition comprising banyan tree, lotus, and clover serum fractions (aging)
  • Composition comprising banyan tree, lotus, and clover serum fractions (aging)
  • Composition comprising banyan tree, lotus, and clover serum fractions (aging)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Example 1 - Exemplary Composition

[0081] Table 1 lists non-limiting examples of compositions of the present invention. These examples are for illustrative purposes only and are not to be construed as limitations of the invention, as many variations thereof are possible without departing from the spirit and scope of the invention, which will be appreciated by those skilled in the art. In these examples, unless otherwise stated, all concentrations are listed in weight percent, and minor materials such as diluents, fillers, etc. may be excluded. Accordingly, the listed formulations include the listed ingredients and any lesser materials associated with those ingredients. It will be apparent to those skilled in the art that the selection of these minor species will vary depending on the physical and chemical characteristics of the particular ingredients selected to practice the invention described herein.

[0082] All examples can be used to treat or improve the app...

Embodiment 2

[0089] Example 2 - Ex vivo tissue analysis

[0090] Individual Ficus, Nelumbo and Clover serum fractions as well as mixtures thereof were evaluated at two different concentrations according to the methods described herein. For aging-related genes, the fold increase / decrease in expression relative to the control was determined and listed in Table 2 below. A positive fold increase indicates the desired upregulation of this gene, resulting in a positive anti-aging benefit.

[0091] Ex vivo tissue method: Skin grafts were collected from surgical waste and treated with activators in medium. Control skin was untreated. Seven days later, punch biopsies were taken for RNA isolation and PCR analysis.

[0092] RT-PCR method: application of Quanta iScript TM Convert purified RNA to cDNA. 500ng of RNA was mixed with iScript and run on a thermocycler according to the kit instructions. Then mix 1uL of the generated cDNA with Quanta Perfecta Master according to the instructions TM...

Embodiment 3

[0102] Example 3 - Keratinocyte analysis of claudin 1 and AQP-3 expression

[0103]Keratinocyte cultures: Various mRNA analyzes were performed. Isolation of RNA from neonatal human keratinocytes. Neonatal human keratinocytes (Cascade Biologics # C-001-5C) were grown to 40% confluency in 6-well tissue culture plates and then treated with compounds for 24 hours (3 replicates per treatment). The treatment consisted of several concentrations of the banyan + clover + lotus mixture as well as the sap alone. Additionally, a vehicle control was applied.

[0104] Several targets were assayed with the Panomics QuantiGene Plex Assay kit, based on the analysis of multiple analyte quantitation beads. For aquaporin 3 and claudin 1, the fluorescence intensity values ​​measured by the Bio-Rad Bio-Plex 100 instrument are shown for those samples that produced significant RNA.

[0105] figure 1 The graphs show that claudin-1 and AQP3 are hopefully upregulated in response to the banyan +...

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PUM

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Abstract

The present invention relates to compositions comprising banyan tree, lotus, and clover serum fractions. A method of improving the appearance of aging skin may comprise the step of applying a composition comprising an effective amount of banyan tree serum fraction, lotus serum fraction, and clover serum fraction to a sign of aging on a skin surface, wherein the composition is applied for a period of time sufficient to improve the appearance of the aging skin. The method may include the step of identifying a sign of aging on a facial skin surface.

Description

field of invention [0001] The present invention relates to compositions comprising serum fractions of banyan tree, lotus and clover. The present invention also relates to a method of improving the appearance of aging mammalian skin using a composition comprising a banyan, nelumbo, and clover serum fraction. Background of the invention [0002] The epidermis, the outermost layer of the skin, consists of a continuum of four cellular layers: the stratum corneum, the granular layer, the spinous layer, and the basal layer. Each cell layer in the epidermis represents various stages along a process in which basal epidermal keratinocytes undergo a continuous cycle of proliferation, differentiation, and apoptosis, moving upward from the basal layer to eventually produce keratinocytes. These corneocytes form the cornified layer, known as the stratum corneum. [0003] Basal keratinocytes are located in the lower part of the epidermis. These actively dividing cells undergo a prolife...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/97A61Q17/00A61Q19/00A61Q19/08
CPCA61K36/48A61K36/60A61Q19/00A61Q17/00A61K8/97A61Q19/08A61K36/185A61K8/9739A61K8/9789A61K8/9794A61P17/00A61P43/00
Inventor M·克格诺夫O·杜瓦-克格诺夫
Owner NOURYON CHEM INT BV
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