New strain of streptomyces thermocarboxydus and application thereof

A carbon monoxide and streptomyces technology, applied in application, bacteria, biochemical equipment and methods, etc., can solve the problems of discarding livestock and poultry manure, waste of resources, environment, pollution, etc. The effect of increased content

Inactive Publication Date: 2014-12-17
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past two decades, a group of more and more intensive farms have been established in the suburbs of cities that are convenient for transportation with the strong support of the government. Discarded in the natural environment at will, resulting in a great waste of resources and environmental pollution

Method used

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  • New strain of streptomyces thermocarboxydus and application thereof
  • New strain of streptomyces thermocarboxydus and application thereof
  • New strain of streptomyces thermocarboxydus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 The preparation of thermophilic streptomyces carbon monoxide bacterial agent

[0026] Inoculate Streptomyces carbonmonoxide thermophilic Streptomyces CF1 on the slant of Gaoshi No. 1 solid medium, and cultivate it at a constant temperature of 50°C for 72 hours to obtain slant seeds;

[0027] The slant seeds were inoculated into Gaoshi No. 1 liquid medium, and cultured at a constant temperature of 50°C and at a speed of 180r / min for 72 hours to obtain liquid seeds;

[0028] Inoculate the liquid seed into the production medium in the liquid fermenter with 5% (V / V, that is, inoculate 5ml liquid seed per 100ml liquid medium) inoculum amount, and carry out strain production. Under continuous aeration, 50°C Cultured at constant temperature for 4 days. The cultured fermented liquid can be used as bacterial agent.

[0029] The composition of the Gaoshi No. 1 solid medium is: add 20.0 g of soluble starch per 1000 ml of distilled water, KNO 3 1.0g, K 2 HPO 4 0...

Embodiment 2

[0032] Mix fresh cow dung and crushed straw at a dry weight ratio of 6:1, or adjust the ratio appropriately according to the physical and chemical properties of cow dung and sawdust, so that the C / N of the mixture is 30 and the water content is 60%.

[0033] The bacteria agent produced in Example 1 was inoculated into the compost mixture according to the mass ratio of 5‰, and the compost was fermented and fermented by regular turning and aeration. Simultaneously, the heap without the bacterial agent of the present invention was used as a control.

[0034] Compared with the cow dung of natural composting, the cow dung inoculated with the microbial agent of the present invention has a fast heating rate, a high maximum temperature of the heap body, a short fermentation period, and a rapid decline in its C / N. The microbial bacterial agent described in the invention can accelerate the maturity of cow dung compost.

[0035] By comparing and analyzing the nutrient content of the pil...

Embodiment 3

[0042] Embodiment 3 is used for the compound bacterial agent of animal excrement fermentation

[0043] Take 10 mL of the overnight cultured Bacillus subtilis seed liquid and transfer it into 500 ml of fermentation medium, and ferment at 28° C. for 20 hours at a rotation speed of 180 rpm.

[0044] The composition of Bacillus subtilis fermentation medium is: sucrose 10g / L, peptone 5g / L, beef extract 3g / L, yeast extract powder 1g / L, magnesium sulfate heptahydrate 0.5g / L, manganese sulfate 0.0005g / L, pH7 .0.

[0045] Take 20mL of the overnight cultured Bacillus megaterium seed solution and transfer it into 500ml of fermentation medium, and culture it at 30°C for 48h. The speed is 180rpm.

[0046] The composition of Bacillus megaterium fermentation medium is: beef extract 5g / L, peptone 5g / L, sodium chloride 5g / L, pH7.0.

[0047] The fermentation broth of Streptomyces carbonmonoxide thermophilus CF1 was prepared according to the method of Example 1.

[0048] The fermented liquid...

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Abstract

The invention discloses a new strain CF1 of streptomyces thermocarboxydus, wherein the collection number is CGMCC (China General Microbiological Culture Collection Center) No.6873, the collection date is November 23, 2012, and the collection unit is CGMCC. The invention further discloses a bacterial agent containing the strain. The new strain CF1 of the streptomyces thermocarboxydus, disclosed by the invention, is inoculated into a compost, so that the temperature of the compost can be rapidly raised in low-temperature seasons in the North, the high temperature of above 50 DEG C of the compost can be maintained for 15 days, the fermentation period is shortened, and pathogens and parasites in animal dung can be effectively killed. After the end of fermentation of the compost, the nutrient content in a composted product is high, the pH value is about 8.0, and the composted product can be used as an excellent plant organic fertilizer.

Description

technical field [0001] The invention belongs to the field of soil fertilizers, and in particular relates to an agricultural microbial preparation and its application. Background technique [0002] With the rapid development of social economy, people's living standards continue to improve, and the dietary structure has undergone tremendous changes. Among them, livestock and poultry products play an indispensable and important role in the dietary structure, thus promoting the rapid development of the livestock and poultry breeding industry. . In the past two decades, a group of more and more intensive farms have been established in the suburbs of cities that are convenient for transportation with the strong support of the government. Discarded in the natural environment due to chemical treatment, resulting in a great waste of resources and environmental pollution. While satisfying people's demand for meat, eggs, and milk, it has brought enormous pressure to the natural ecolo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08C05F17/00C12R1/465C12R1/11C12R1/125
CPCY02W30/40
Inventor 刘克锋王顺利王红利高程达石爱平
Owner BEIJING UNIV OF AGRI
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