Micro-ecological preparation and application thereof in improvement of production performance, immune performance as well as chicken quality of broiler
A technology of microecological preparations and broiler chickens, applied in the direction of microorganism-based methods, applications, microorganisms, etc., can solve problems such as adverse effects on the ecological environment, threats to human health and safety, and decline in cellular and humoral immune functions of broilers
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Embodiment 1
[0075] Embodiment 1, the preparation of composite bacterial agent for broilers
[0076] This example relates to two kinds of composite bacterial agents for broilers, which are composite bacterial agents A for broilers and composite bacterial agents B for broilers.
[0077] Composite microbial agent A for broilers is to dilute the liquid strains of Bacillus subtilis RJGP16, Saccharomyces cerevisiae CICC1562 and Lactobacillus plantarum CICC22696 to 10 8 After cfu / ml, the resulting mixture was mixed according to the colony-forming unit number ratio of 1:3:1.
[0078] The compound bacterial agent B for broilers is to dilute the liquid strains of Bacillus subtilis RJGP16, Saccharomyces cerevisiae CICC1562 and Lactobacillus plantarum CICC22696 to 10% respectively with sterile water. 8 After cfu / ml, the resulting mixture was mixed according to the colony-forming unit number ratio of 1:1:1.
[0079] Wherein, the liquid strain of Bacillus subtilis RJGP16 is a culture obtained by cult...
Embodiment 2
[0083] Embodiment 2, the preparation of broiler probiotics
[0084] This example provides two kinds of probiotics for broiler chickens, respectively called probiotics A for broilers and probiotics B for broilers.
[0085] The probiotics A for broilers is a culture obtained by using the fermentation medium A of Example 1 to cultivate the composite microbial agent A for broilers of Example 1. The specific preparation method is as follows: the broiler composite bacterial agent A of embodiment 1 is inserted into the 200L embodiment according to the inoculum amount (the volume of the composite bacterial agent inserted and the volume ratio of the fermentation medium) of 6% (volume ratio). In the 500L fermenter of the fermentation medium A of 1, cultivate for 42 hours at 34°C, with an aeration rate of 600L / h and a stirring speed of 150rpm, collect all the cultured substances (fermentation liquid) in the fermenter, and add The rice husk powder was dried at 50° C. to obtain the probio...
Embodiment 3
[0087] Embodiment 3, improve broiler body weight with the broiler chicken probiotic A of embodiment 2
[0088] 1.1 Test animals
[0089] Select 12,000 1-day-old healthy chicks of Arboriga—Quick large white-feathered broiler chicken with a body weight of 50±5 grams, and divide them into 4 treatment groups at random, namely, the control group, the 0.015% probiotics group, and the 0.025% probiotics group. Ecological preparation group and 0.05% probiotic preparation group. There were 3 replicates per treatment group with 1000 chickens per replicate.
[0090] 1.2 Diet
[0091] Broiler brooding period diets were divided into broiler brooding period common rations, 0.015% broiler probiotics A brooding rations, 0.025% broiler probiotics A brooding rations, 0.05% broiler probiotics A brooding rations. Among them, the common diet for broiler brooding period is shown in Table 1; 0.015% broiler chicken probiotics A brooding period diet is added to the common diet for broiler chicken br...
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