Preparation of zirconium dioxide and ferriferrous oxide nanoparticles and method used for enrichment of phosphopeptides by using zirconium dioxide and ferriferrous oxide nanoparticles
A technology of ferric tetroxide and enrichment of phosphopeptides, which is applied in the field of nanomaterials and protein separation and purification, can solve the problems of numerous steps, short production cycle, difficult enrichment of phosphopeptides, etc., and achieves short enrichment cycle, simple operation, The effect of mild reaction conditions
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[0040] 1. Preparation of Zirconium Dioxide Ferric Oxide Magnetic Nanoparticles
[0041] Synthesized by chemical co-precipitation method, the chemical reaction equation is:
[0042] Fe 2+ +2Fe 3+ +8NH 3 ·H 2 O=Fe 3 o 4 ↓+8NH 4+ +4H 2 o
[0043] Proceed as follows:
[0044] (1) the soluble Fe 2+ and Fe 3+ Dissolve in water-ethanol mixed solution (v / v=10:1), and pass nitrogen gas for 20min under mechanical stirring.
[0045] (2) Raise the temperature to 60° C., slowly drop ammonia solution into the system under stirring, adjust the pH value of the system to about 10, and obtain a black solution containing magnetic particles.
[0046] (3) Rapidly add 20% zirconium oxychloride solution under stirring state, raise the temperature to 80° C. and react for 1 hour, terminate the reaction, and let it stand for cooling to obtain a reaction solution.
[0047] (4) The magnetic particles are separated under the action of an external magnetic field, and washed several times with de...
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