Non-isotonic preserving fluid for liquid-based thin-layer exfoliative cells

Abstract

The invention discloses a non-isotonic preserving fluid for liquid-based thin-layer exfoliative cells, and is characterized by comprising the following raw materials by weight: 9-11 parts of methyl aldehyde, 13-16 parts of ethyl alcohol, 9-9.5 parts of glycine, 3-3.5 parts of carbamide, 1.8-2.2 parts of procaine hydrochloride, 1.3-1.6 parts of propylene glycol, 1.4-1.6 parts of dimethyl sulfoxide, 0.8-1.2 parts of sodium phosphate, 3.4-3.6 parts of trypsin, 0.1-0.3 part of acetic acid, 1-1.1 parts of disodium hydrogen phosphate, 0.3-0.32 part of sodium dihydrogen phosphate, and 51-52 parts of water. Compared with the prior art, the non-isotonic preserving fluid has the advantages as follows: buffer solution fixed cells by the formula of the non-isotonic preserving fluid facilitate histochemical stain; the solution utilizing the colligative property of a non-ionic solution splits red blood cells and dissolves mucus protein during the hydroformylation process, so that the microscopic examination background becomes clear, and the detection rate of positive cells is greatly improved.

Description

technical field [0001] The invention relates to a preservation solution for dilution, cell fixation, mucolysis, bacteriostasis and preservation of cervical scrapes, pleural ascites, sputum exfoliated cells and the like for liquid-based thin-layer exfoliated cell detection in medical clinical examinations. Background technique [0002] At present, there are many kinds of specimen preservation solutions for exfoliated cells such as cervical scrapes for liquid-based thin-layer cell detection in medical clinical examinations, but the main functions of these products are: (1) Dissolve mucin to prevent wrapping positive cells; (2) ) Lysate red blood cells to reduce background cells and make the background clear for staining microscopy; (3) Fix white blood cells, epithelial cells and histiocytes; (4) Maintain neutral pH for Papanicolaou or histochemical staining. The main components and functions of the current mainstream product liquid-based thin-layer cell preservation solution a...

AI Technical Summary

Problems solved by technology

The main component and function of the current mainstream product liquid-based thin-layer cell preservation solution is to use alcohol as a cell fixative and red blood cell hemolysis agent, but the concentration requirement is very high, reaching 95%, which brings many problems: (1) High concentration of alcohol Alcohols cause rapid coagulation of proteins and mucus proteins, and envelop positive cells, which brings great difficulties to mucolytic treatment; (2) Alcohols are non-aqueous media, which weakens the buffering capacity of the acid-base buffer system of the solution; (3) If alcohols The concentration of 30-40% can not fix the cells; (4) fix the cells with 10% formaldehyde, but after the erythrocytes are hydroformylated, the current formula of liquid-based thin-layer cell preservation solution cannot crack the erythrocytes that have been hydrolyzed. Increase the background cells of the smear, increase the difficulty of microscopic examination and the risk of false negative determination

[0004] The alcohol liquid-based thin-layer exfoliated cell preservation solution used in the market, the alcohol concentration of 30-40% cannot fix the cells; the alcohol concentration of 95% can fix the cells, but this high Concentration alcohol non-aqueous solution makes the acid-base buffering capacity of the liquid-based thin-layer exfoliated cell preservation solution lose