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Locust microspore chorthippus strain and applications thereof

A technology of microspores and locusts, applied in applications, microorganisms, pesticides, etc., can solve the problems of high temperature and ultraviolet resistance, poor stress resistance, etc., and achieve the effects of strong ultraviolet resistance, reduced residue, and simple production technology

Active Publication Date: 2013-12-18
HEBEI SHENGPU BIOLOGICAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the locust microspores currently used in demonstration applications are imported from the United States and belong to foreign species, and there are certain ecological risks after application; in addition, the existing locust microspore strains have poor stress resistance, especially the ability to resist high temperature and ultraviolet rays

Method used

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  • Locust microspore chorthippus strain and applications thereof
  • Locust microspore chorthippus strain and applications thereof
  • Locust microspore chorthippus strain and applications thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0053] Example 1. Acquisition and Identification of Paranosema acridophagus PA-201301

[0054] 1. Acquisition of Paranosema acridophagus strain PA-201301

[0055] Dissect the infected young locusts collected from the wild on the Qinghai-Tibet Plateau to detect the infection by microspores, put the intestinal tract into a homogenizer to homogenate, filter with medical nylon gauze (40-60 mesh), and use Centrifuge at a centrifugal force of 6000g-8000g for 12 minutes to obtain different microspore strains. One of the microspore strains isolated from C. stenoptera was designated as PA-201301 strain.

[0056] 2. Identification of Paranosema acridophagus strain PA-201301

[0057] In the systematic classification of microspores, features such as spore morphology, size, pathogenicity, number of pole filament turns and pole filament angle are the main features of identification. The inventors of the present invention compared and tested the PA-201301 strain obtained in step 1 Compare...

Embodiment 2

[0073] Example 2. Application of Paranosema acridophagus PA-201301 in controlling locusts

[0074] 1. Proliferation and culture of Paranosema acridophagus PA-201301

[0075] For the cultivation process flow chart of Paranosema acridophagus PA-201301 CGMCC No. 7757, see figure 1 .

[0076] 1. Spore proliferation

[0077] Determination of the proliferation of paranosema acridophagus (Paranosema acridophagus) PA-201301CGMCC No.7757 in the endosomes of the following 7 species of locusts: Stenophora spp. White-sided scab and cloud-spotted grasshopper.

[0078] (1) Hatch the eggs of locusts as hosts at 20-30°C; feed fresh corn leaves every day at 28-32°C and relative humidity of 60-70% until the 4th to 5th instar.

[0079] (2) Take 20 μL of the spore liquid of Paranosema acridophagus PA-201301CGMCC No.7757 (1×10 6 spores / mL) were injected into the oral cavity of the 4-5 instar locusts obtained in step (1), and inoculated once. After inoculation, feed with fresh corn leaves unt...

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Abstract

The invention discloses a chorthippus microspore strain and applications thereof. The chorthippus microspore strain disclosed by the invention is paranosema acridophagus PA-201301 with the preservation number of CGMCC No.7757 in the China General Microbiological Culture Collection Center. The paranosema acridophagus PA-201301 CGMCC No.7757 disclosed by the invention has the advantages of obvious lethal effect to chorthippus dubius, chorthippus fallax, myrmeleotettix palpalis, calliptamus abbreviatus, bryodema luctuosum, oriental migratory locust and gastrimargus marmoratus than that of an existing locust microspore, and stress resistance, in particular strong ultraviolet resistance, wherein the germination rate of spores is still more than 80% after a spore solution is irradiated by the ultraviolet light for one hour; in addition, the production technology of microspores of the chorthippus microspore strain is simple, and propagation hosts are easily obtained; after homogenating, separation is performed at the centrifugal force of about 6000g; the chorthippus microspore strain disclosed by the invention is conductive to mass production and applications.

Description

technical field [0001] The present invention relates to a new strain of microspore locust and its application, in particular to a microspore young locust strain of locust and its application in controlling locust Background technique [0002] The locust plague is a worldwide natural disaster, which has had a great impact on agricultural production. Over the years, there have been frequent outbreaks of locust plagues in large areas, including soil locusts, migratory locusts, rice locusts, cotton locusts and bamboo locusts. Only migratory locusts and soil locusts occur on 25 million mu and 70 million mu each year, seriously affecting agricultural and animal husbandry production. [0003] Since the 1970s, the United States used black locusts as breeding hosts to cultivate locust microspores, and then domestic and foreign studies on locust microspores have been carried out one after another. However, the locust microspores currently used in demonstration applications are impor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/10A01N63/00A01P7/04C12R1/90
Inventor 张英霞
Owner HEBEI SHENGPU BIOLOGICAL SCI & TECH CO LTD
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