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Extraction and thin layer chromatographic scanning detection method for defatted Euphausia superb saponin

An Antarctic krill and thin-layer chromatography technology, which is applied in measurement devices, analytical materials, instruments, etc., to achieve the effect of simple detection methods

Inactive Publication Date: 2013-12-11
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on the extraction of Antarctic krill saponins. The present invention extracts Antarctic krill saponins and detects and quantifies them

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Add 100g of degreased dried Antarctic krill to 600mL of methanol, heat and stir in a water bath at 80 degrees Celsius to extract 3 times, and filter after each extraction for 30 minutes. The extracts were combined, and the methanol was removed by rotary evaporation to obtain 60 g of paste. Add 360 mL of diethyl ether to the paste, stir to dissolve, and filter off the sediment to obtain the filtrate. The diethyl ether in the filtrate was evaporated to obtain 15 g of saponin crude extract.

[0025] The saponin-containing crude extract was dissolved in methanol to prepare a sample solution with a concentration of 30 μg / μL. Oleanolic acid was prepared into five different concentrations of standard solutions: 0.6, 2.4, 3.0, 3.6, 4.2 μg / μL.

[0026] Spot the sample solution (3μL / point) and standard solution (1μL / point) on the same piece of GF 254 High-efficiency thin-layer silica gel plate (10×10cm 2 )superior. The developer is xylene-butyl acetate-glacial acetic acid, t...

Embodiment 2

[0028] The difference with Example 1 is: the paste obtained is 61g, and ether 488ml is added to the paste to obtain 15.3g of saponin crude extract; the volume ratio of xylene-butyl acetate-glacial acetic acid in the developer is 10 :3:0.4, concentration is 10% sulfuric acid ethanol solution volume is 10ml, and other steps are identical with embodiment 1, use gained relational regression equation in embodiment 1, the content that gets saponin is 0.17g / 100g.

Embodiment 3

[0030] The difference with Example 1 is: the paste obtained is 62g, and ether 620ml is added to the paste to obtain 15.5g of saponin crude extract; the volume ratio of xylene-butyl acetate-glacial acetic acid in the developer is 11 :5:0.3, concentration is that the sulfuric acid ethanol solution volume of 10% is 10ml, and other steps are identical with embodiment 1, use gained relational regression equation in embodiment 1, the content that gets saponin is 0.17g / 100g.

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PUM

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Abstract

The invention discloses an extraction and thin layer chromatographic scanning detection method for defatted Euphausia superb saponin. The method specifically comprises the following main steps: extracting defatted Euphausia superb saponin and performing thin layer chromatographic scanning detection. According to the extraction method and thin layer chromatographic scanning detection method for defatted Euphausia superb saponin provided by the invention, the detection method is simple, rapid, accurate, reliable and stable.

Description

technical field [0001] The invention relates to the extraction and thin layer chromatography scanning detection method of defatted Antarctic krill saponin. Background technique [0002] Antarctic krill (Euphausia superba) is a kind of krill that lives in the waters of Antarctica. It lives in clusters, sometimes with a density of 10,000 to 30,000 per cubic meter. They feed on tiny phytoplankton, are about 6 cm long, weigh about 2 grams, and have a lifespan of 6 years. They are a keystone species in the Antarctic ecosystem and probably the most successful living species on Earth in terms of biomass (approximately 500 million tonnes). Because of the huge reserves of Antarctic krill and the fact that the population is easy to catch, many countries are attracted to develop it. At present, the most important use of Antarctic krill is as fish feed. In Norway, Canada and other countries, Antarctic krill shrimp oil is extracted as a health product, and the price is about 2,000 RMB...

Claims

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Application Information

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IPC IPC(8): G01N30/90
Inventor 王翩翩刘代成
Owner SHANDONG NORMAL UNIV
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