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Method for quickly determining residual peracetic acid

A peracetic acid, rapid determination technology, applied in the field of biotechnology enzymatic analysis, can solve the problems of lack of detection of peracetic acid residues, etc., and achieve the effects of saving test costs, fast response time, and good stability

Active Publication Date: 2013-12-11
GUANGDONG HUANKAI MICROBIAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is still a lack of widely recognized methods for the detection of peracetic acid residues, so it is imminent to develop a convenient, high-sensitivity, and reliable peracetic acid residue testing product

Method used

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  • Method for quickly determining residual peracetic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Prepare with pure water containing 0.04g / L horseradish peroxidase, 1.5g / L TMB hydrochloride, 0.037mol / L citric acid, 0.126mol / L disodium hydrogen phosphate, 10 g / L trehalose, 5 g A mixed chromogenic solution of / L glycine, 3g / LBSA, 10g / LPEG4000. Soak the chromatographic filter paper completely in the above mixture for about 10 minutes, take out the filter paper, and dry it in vacuum at a temperature lower than 30°C. Cut the filter paper into small pieces of 5×6 mm and glue them on a strip of 5×80 mm One end of the base plate is made into peracetic acid residue rapid determination test paper 1#.

Embodiment 2

[0038] Prepare with pure water containing 0.01g / L horseradish peroxidase, 1.0g / L TMB sulfate, 0.028mol / L citric acid, 0.11mol / L disodium hydrogen phosphate, 10g / L trehalose, 5g / L essence Amino acid, 5g / LBSA, 10g / LPVP mixed chromogenic solution. Soak the chromatographic filter paper completely in the above mixture for 5 minutes, take out the filter paper, and dry it under vacuum at a temperature lower than 30°C. Cut the filter paper into small pieces of 5×5 mm and glue them on a One end of the bottom plate is made of peracetic acid residue rapid determination test paper 2#.

Embodiment 3

[0040] Prepare with pure water containing 0.025g / L horseradish peroxidase, 1.25g / L TMB propanesulfonate, 0.044mol / L citric acid, 0.14mol / L disodium hydrogen phosphate, 2g / L sucrose, 2g / L Mixed chromogenic solution of trehalose, 2g / L glycine, 1g / LHSA, 4g / LPEG6000. Completely immerse the qualitative in the above mixture for about 20 minutes, take out the filter paper, and dry it in vacuum at a temperature lower than 30°C. Cut the filter paper into small pieces of 5×5 mm, and glue them on a 5×50mm strip of plastic One end of the hard board is made of peracetic acid residue rapid determination test paper 3#.

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Abstract

The invention discloses a method for quickly determining residual peracetic acid. The method is short in response time, high in sensitivity, good in stability, easy and simple to operate, economical and practical, and can quickly detect the content of residual peracetic acid in a sample on site; the lowest detected content of peracetic acid is 0.5mg / L; a pH modifier in test paper used by the method ensures the accuracy of a test result; an enzyme protecting agent in the test paper ensures the stability of the enzyme test paper; a guarantee period can at least reach 12 months; the used test paper is small in area; a test process is simple; other instruments or accessories are not required; the test cost can be saved greatly; a holding bottom plate of the test paper can be prevented from being polluted by a hand in a taking process; and the method is very safe and reliable.

Description

technical field [0001] The invention belongs to the field of biotechnology enzymatic analysis, in particular to a rapid determination method for peracetic acid residue. Background technique [0002] As a new type of high-efficiency and low-toxicity bactericidal disinfectant, peracetic acid is mainly used in the disinfection of food processing equipment, medical equipment and supplies. With the deterioration of the environment, there are more and more kidney disease patients in the world. According to incomplete statistics, the number of hemodialysis patients in my country has exceeded 100,000 in 2008, so the disinfection of peracetic acid in hemodialysis is particularly important. The hemodialysis machine generally needs to be disinfected once every 1-3 months, and 0.2% peracetic acid can be used to circulate for 20 minutes and soak for 30 minutes to disinfect; the water storage tank and pipeline generally need to be disinfected once every 15 days, and 0.2% peracetic acid ca...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 邓金花秦惠黄报亮吴清平
Owner GUANGDONG HUANKAI MICROBIAL SCI & TECH
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