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Technological condition for preparing antihypertensive peptide from northeast China juglans mandshurica maxim kernel protein by employing enzymatic method

A blood pressure-lowering peptide, an enzymatic preparation technology, applied in the field of enzymatic preparation of Northeast walnut catalpa seed protein blood pressure-lowering peptide, to achieve the effect of simple process operation, cost saving, and good resolubility

Inactive Publication Date: 2013-12-04
NORTHEAST FORESTRY UNIVERSITY +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on the preparation of antihypertensive peptides from the kernel protein of the Northeast Juglans catalpa

Method used

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  • Technological condition for preparing antihypertensive peptide from northeast China juglans mandshurica maxim kernel protein by employing enzymatic method
  • Technological condition for preparing antihypertensive peptide from northeast China juglans mandshurica maxim kernel protein by employing enzymatic method
  • Technological condition for preparing antihypertensive peptide from northeast China juglans mandshurica maxim kernel protein by employing enzymatic method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Add alkaline protease to 100ml of 2% Northeast Juglans catalpa seed protein solution, enzymolysis at 50°C for 3 hours, the amount of enzyme added is 5000U / g substrate, the pH of enzymolysis is 8, and the enzymolysis process is adjusted with 0.1mol / L NaOH solution The corresponding pH was controlled, and after enzymolysis, the enzyme was inactivated in a boiling water bath at 100°C for 15 minutes; the enzymolysis solution was centrifuged at 4000r / min for 10 minutes, the degree of hydrolysis of the supernatant was 25.784%, and the ACE inhibition rate was 61.135%. The supernatant was subjected to ultrafiltration under the conditions of a pressure of 0.2 MPa, a temperature of 30° C., a permeate flow rate of 2.5 L / h, and a molecular weight intercepted by ultrafiltration of 5000 Da. The Northeast Juglans catalpa seed protein hypotensive peptide obtained after the permeate is concentrated and freeze-dried is a finished product in the form of light yellowish brown powder.

[00...

Embodiment 2

[0043] Add alkaline protease to 100ml of 2% Northeast Juglans catalpa seed protein solution, enzymolysis at 50°C for 4 hours, the amount of enzyme added is 5000U / g substrate, the pH of enzymolysis is 8, and the enzymolysis process is adjusted with 0.1mol / L NaOH solution The corresponding pH was controlled, and after enzymolysis, the enzyme was inactivated in a boiling water bath at 100°C for 15 minutes; the enzymolysis solution was centrifuged at 4000r / min for 10 minutes, and the degree of hydrolysis of the supernatant was 27.567%, and the ACE inhibition rate was 62.375%. The supernatant was subjected to ultrafiltration under the conditions of a pressure of 0.2 MPa, a temperature of 30° C., a permeate flow rate of 2.5 L / h, and a molecular weight intercepted by ultrafiltration of 5000 Da. The Northeast Juglans catalpa seed protein hypotensive peptide obtained after the permeate is concentrated and freeze-dried is a finished product in the form of light yellowish brown powder.

Embodiment 3

[0045] Add alkaline protease to 100ml of 2% Chinese catalpa seed protein solution, add alkaline protease, and enzymolyze at 56°C for 4 hours, the amount of enzyme added is 5880U / g substrate, the pH of enzymolysis is 8.2, and the enzymolysis process is adjusted with 0.1mol / L NaOH solution The corresponding pH was controlled, and after enzymolysis, the enzyme was inactivated in a boiling water bath at 100°C for 15 minutes; the enzymolysis solution was centrifuged at 4000r / min for 10 minutes, the degree of hydrolysis of the supernatant was 29.03%, and the ACE inhibition rate was 72.48%. The supernatant was subjected to ultrafiltration under the conditions of a pressure of 0.2 MPa, a temperature of 30° C., a permeate flow rate of 2.5 L / h, and a molecular weight intercepted by ultrafiltration of 5000 Da. The Northeast Juglans catalpa seed protein hypotensive peptide obtained after the permeate is concentrated and freeze-dried is a finished product in the form of light yellowish brow...

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Abstract

The invention relates to a technological condition for preparing an antihypertensive peptide from northeast China juglans mandshurica maxim kernel protein by employing an enzymatic method. The main steps comprise: performing peel-removing, crushing and degreasing on northeast China juglans mandshurica maxim kernels, preparing kernel protein by employing an alkali-extraction acid-precipitation method; preparing a northeast China juglans mandshurica maxim kernel protein solution with a concentration of 2%, performing enzymolysis on the protein solution with alkali protease to prepare the antihypertensive peptide, paying attention to corresponding conditions during enzymolysis, after enzymolysis killing the enzyme by employing a boiling water bath; centrifuging zymolyte, performing ultrafiltration on the supernatant ( with molecular weight cutoff of 5000 Da), concentrating the penetrated liquid, performing vacuum freeze drying to obtain the northeast China juglans mandshurica maxim kernel protein antihypertensive peptide. According to the technological condition, the northeast China juglans mandshurica maxim kernel protein is taken as the raw material and the enzymatic method is employed for preparation of a polypeptide with functions of reducing blood pressure; the technological condition lays a foundation for deep processing and utilization on juglans mandshurica maxim; and also the technological condition is simple and applicable to industrialized production.

Description

technical field [0001] The invention relates to a process condition for preparing the blood pressure-lowering peptide from the Northeast Juglans catalpa seed protein by enzymatic method. Background technique [0002] Walnut catalpa (Juglans Mandshurica Maxim) is produced in the Heilongjiang Xiaoxing'an Mountains below an altitude of 500-800m, and the eastern mountainous areas, Jilin, Liaoning, eastern Inner Mongolia, Hebei, Shanxi and other places. Walnut catalpa seed kernels are rich in protein, a variety of essential fatty acids and 22 kinds of mineral elements such as calcium, magnesium, phosphorus, zinc and iron that are necessary for the human body. Its protein digestibility reaches 87.2%, 18 kinds of amino acids are complete, and the proportion of 8 kinds of essential amino acids required by the human body is suitable, and the content of glutamic acid, aspartic acid and arginine, which have important functions in human physiology, is relatively high , so walnut protei...

Claims

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Application Information

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IPC IPC(8): C12P21/06
CPCY02P20/54
Inventor 包怡红于阳阳
Owner NORTHEAST FORESTRY UNIVERSITY
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