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Preparation method of effective part of Hippophae rhamnoides

A technology of effective parts and sea buckthorn is applied in the field of preparation of the effective parts of Central Asian sea buckthorn to achieve the effects of high safety, good antioxidant and antibacterial activities

Inactive Publication Date: 2013-12-04
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, the content of triterpenoids in seabuckthorn fruit is relatively high (2%-3%), and has the activities of protecting the liver, anti-inflammation, anti-oxidation, antibacterial, and treating ulcers similar to total phenols. Research on the preparation of such components in

Method used

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  • Preparation method of effective part of Hippophae rhamnoides
  • Preparation method of effective part of Hippophae rhamnoides
  • Preparation method of effective part of Hippophae rhamnoides

Examples

Experimental program
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Effect test

Embodiment 1

[0052] a. Dry the seabuckthorn fruit in the shade, crush it, pass it through a 40-mesh sieve, take 100 g of the crushed fruit powder, use 50% ethanol with a concentration of 5 times the mass-volume ratio, heat and reflux in a water bath, and extract 3 times for 3 hours at a water bath temperature of 95°C , collecting the extract, and recovering ethanol under reduced pressure;

[0053] b. Use a vacuum rotary evaporator to concentrate the extract from step a to a mass concentration of 0.067g crude drug / mL at a temperature of 45°C, and use saturated NaHCO 3 Adjust the pH of the solution to 6, then purify the concentrated solution with macroporous resin AB-8, then elute with water and ethanol with a volume concentration of 70% in sequence, collect the ethanol eluate, wherein the elution volume of water is 3BV, and wash with water The flow rate is 1.5BV / h, the ethanol elution volume is 3BV, and the ethanol elution rate is 2BV / h;

[0054] c. Vacuum-dry the ethanol eluate in step b ...

Embodiment 2

[0056] a. Dry the seabuckthorn fruit in the shade, crush it, pass it through a 50-mesh sieve, take 100 g of the crushed fruit powder, use 50% ethanol with a concentration of 10 times the mass-volume ratio, heat and reflux in a water bath, the temperature of the water bath is 80°C, extract twice for 2 hours , collecting the extract, and recovering ethanol under reduced pressure;

[0057] b. Use a vacuum rotary evaporator to concentrate the extract from step a at a temperature of 45°C to a mass concentration of 0.067g crude drug / mL, keep the pH of the original solution at 3.1, then purify the concentrate with macroporous resin HPD-300, and then use water and volume The ethanol with a concentration of 50% was eluted sequentially, and the ethanol eluate was collected, wherein the water elution volume was 3BV, the water washing flow rate was 2BV / h, the ethanol elution volume was 2.5BV, and the ethanol elution rate was 1BV / h;

[0058] c. Vacuum-dry the ethanol eluate in step b to ob...

Embodiment 3

[0060] a. Dry the seabuckthorn fruit in the shade, crush it, pass it through a 40-mesh sieve, take 100 g of the crushed fruit powder, and use 30% ethanol with a concentration of 15 times the mass-volume ratio to heat and reflux in a water bath. The temperature of the water bath is 70 ° C. Extract 3 times for 1 hour , collecting the extract, and recovering ethanol under reduced pressure;

[0061] b. Use a vacuum rotary evaporator to concentrate the extract from step a at a temperature of 45°C to a mass concentration of 0.067g crude drug / mL, and use Na 2 CO 3 Adjust the pH of the solution to 5, then purify the concentrated solution with macroporous resin HPD-450, then elute with water and ethanol with a volume concentration of 30% in sequence, collect the ethanol eluate, wherein the elution volume of water is 3BV, wash with water The flow rate is 3BV / h, the ethanol elution volume is 4BV, and the ethanol elution rate is 4BV / h;

[0062] c. Vacuum-dry the ethanol eluate in step b...

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Abstract

The invention relates to a preparation method of the effective part of Hippophae rhamnoides. The method includes: crushing and screening aired Hippophae fruits or post-pressing Hippophae fruit residue, heating ethanol for reflux extraction, collecting extract, performing reduced-pressure recovery on the ethanol, condensing, enriching the condensate with macroporous resin, and vacuum-drying to obtain the total effective part of Hippophae rhamnoides. Hippophae rhamnoides contains 75-85% of the total effective part. The total effective part contains 35-50% of triterpene and 32-45% of total phenols. The results of in-vitro anti-oxidization and anti-bacteria experiments and bioactivity experiments show that the effective part of Hippophae rhamnoides is high in capacity of clearing free radicals in 2, 2-azino-bis(3-ethyl- benzothiazole-6-sulfonate) diammonium salt, high in inhibition on Staphylococcus aureus, and high in oxidation resistance and gram-positive bacterium inhibition. The product obtained by the preparation method is applicable to health foods, drugs, cosmetics or solid drink materials having the functions of protecting liver, lowering blood lipid, age defying, protecting gastric mucosa, antisepsis and anti-inflammation.

Description

technical field [0001] The invention relates to a preparation method of the effective part of seabuckthorn from Central Asia. Background technique [0002] Seabuckthorn is an economic crop with great development potential, which has very superior ecological and social benefits. Not only has the ecological function of windbreak, sand fixation and soil and water conservation, but also because it is rich in bioactive ingredients such as vitamins, flavonoids, organic acids, terpenoids, sterols, carotenoids, unsaturated fatty acids, etc., it is widely used in cosmetics, health food and medicine. It has very good development value. my country has a long history of medicinal use of seabuckthorn. The medicinal effect of seabuckthorn is recorded in detail in the Tibetan medical book "Yuewang Yaozheng" in the eighth century AD, which is the earliest medical record about seabuckthorn in the world. In the traditional medicine of Uighur medicine, Tibetan medicine and Mongolian medicine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P1/16A61P3/06A61P39/06A61P1/00A61P29/00A61P31/04A23L1/29A61K8/97A23L2/00A61Q19/08A23L33/00
Inventor 阿布力米提·伊力李寅庆阿吉艾克拜尔·艾萨
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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