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Sea cucumber extract and application thereof

A technology of extract and sea cucumber, which is applied to sea cucumber extract and its application field to achieve the effects of improving learning and memory ability, reducing malondialdehyde content and reducing lipid peroxidation

Inactive Publication Date: 2013-12-04
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on AD from different sea cucumber active substances.

Method used

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  • Sea cucumber extract and application thereof
  • Sea cucumber extract and application thereof
  • Sea cucumber extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Preparation of sea cucumber extract and its purified product

[0023] 1. Materials and methods

[0024] After the sea cucumber is dried, the sample is crushed, 10 kg of the sample is taken, and 50L of 95% ethanol solution is ultrasonically assisted countercurrent extraction for 4 h. After filtration, it was concentrated under reduced pressure to 500 mL, and 2L of acetone was added to mix well, and placed at -20°C for 24 h. The precipitate was collected and concentrated under reduced pressure to remove organic solvents to obtain sea cucumber extract.

[0025] First, the sea cucumber extract is dissolved in chloroform, and then the chloroform solution of the sea cucumber extract is poured into the activated silica gel chromatography column, and the flow rate is controlled to 1 mL / min to make the sea cucumber extract and the silica gel fully adsorb. After loading the sample, elute with 5 column volumes of chloroform and acetone in sequence; after washing with acetone, ...

Embodiment 2

[0030] Example 2: The effect of sea cucumber extract and its purification on the learning and memory abilities of SAMP8 mice

[0031] 1. Materials and methods

[0032] 1) Sample preparation

[0033] The preparation scheme of sea cucumber extract and its purified product is the same as in Example 1.

[0034] 2) Experimental animals and groups

[0035] 30 healthy male 1-month-old SAMP8 mice and 10 SAMR1 mice, weighing (28±2) g, were provided by the Animal Center. After the mice were adaptively fed for 1 week, the SAMP8 mice were randomly divided into three groups according to their body weight: the model group, the sea cucumber extract group and the purified product group, each with 10 mice, and the SAMR1 mice under the same conditions were used as Control group. The feed was improved with reference to the AIN-93G rodent feed formula. The sea cucumber extract group and the purified group were supplemented with 0.5% sea cucumber extract or purified respectively. All mice were raised in...

Embodiment 3

[0048] Example 3: The effect of sea cucumber extract and its purified substance on the content of malondialdehyde in brain tissue

[0049] 1. Materials and methods

[0050] 1) Sample preparation

[0051] The preparation scheme of sea cucumber extract and its purified product is the same as in Example 1.

[0052] 2) Laboratory animals and groups

[0053] The animals required for the experiment and the grouping scheme are the same as in Example 2.

[0054] 3) Experimental method

[0055] The experimental mice were decapitated and sacrificed after 4 months of continuous feeding. The brain tissue was immediately taken out, homogenized, centrifuged at 4°C at 4000 rpm for 15 min, and the supernatant was taken. The content of malondialdehyde in the brain tissue of each group of mice was determined by using a malondialdehyde detection kit.

[0056] 4) Statistics

[0057] All data are analyzed by ONE WAY ANOVA using SPSS 17.0 statistical software, and the data are expressed as M±SD and P <0.05 ind...

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PUM

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Abstract

The invention provides an application of a sea cucumber extract in preparation of products for treating and preventing neurodegenerative diseases such as senile dementia, and provides a novel active substance which is used for improving the neurodegenerative diseases such as the senile dementia, the sea cucumber extract and the application of the sea cucumber extract can sufficiently improve the neurodegenerative diseases such as the senile dementia without causing any toxic and side effect. The sea cucumber extract and a purification product of the sea cucumber extract can improve the learning and memory ability of a senescence-accelerated mouse SAMP8, can reduce malondialdehyde content in the brain tissue of the mouse SAMP8, and reduce lipid peroxidation. The result shows that the sea cucumber extract has the utilization potential in preparing the products for treating and preventing the neurodegenerative diseases.

Description

Technical field [0001] The present invention relates to a new use of sea cucumber extract, specifically the use of sea cucumber extract to prevent, improve and treat neurological diseases such as Alzheimer's disease in the research and development of health food, functional food or medicine. Background technique [0002] Alzheimer’s disease (AD), also known as Alzheimer’s, is a primary neurodegenerative disease that often occurs in the elderly. The clinical manifestations are progressive memory loss and cognitive impairment. The incidence of AD is closely related to age. With the aging of society, the elderly population gradually increases, and the prevalence is on the rise. Studies have shown that β-amyloid peptide (Aβ) is a characteristic pathological change of Alzheimer's disease—an important component of senile plaques, which is produced by the metabolism of β-amyloid precursor protein (APP). [0003] The rapid aging mouse (SAM) is a series of natural rapid aging model animals...

Claims

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Application Information

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IPC IPC(8): A61K35/56A23L1/29A61P25/28A23L33/00A61K35/616
Inventor 武风娟王玉明薛勇李兆杰王静凤常耀光徐杰唐庆娟薛长湖
Owner OCEAN UNIV OF CHINA
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