Method for capturing tumour cells by utilizing microflow chip

Abstract

The invention discloses a method for capturing tumour cells by utilizing a microflow chip. The method for capturing the tumour cells by utilizing the microflow chip comprises the following steps of: preparing cell sap, namely preparing a sample containing tumour cells into a phosphate buffer solution; preflushing, namely preflushing an injection pipeline by adopting a phosphate solution containing no tumour cell; capturing the tumour cells, namely causing the prepared cell sap to flow to the microflow chip through the injection pipeline at a flow rate of 90-110 micron litre per hour, and capturing the tumour cells into the microflow chip. According to the method for capturing the tumour cells by utilizing the microflow chip, the tumour cells can be effectively captured and sorted, the captured tumour cells have good activity, and the tumour cells can be directly analyzed after being captured.

Description

technical field [0001] The invention relates to a method for capturing tumor cells, in particular to a method for capturing tumor cells by using a microfluidic chip. Background technique [0002] Circulating tumor cells usually refer to the cells that come from the primary tumor tissue and enter the peripheral blood of the human body after shedding and infecting. Therefore, circulating tumor cells can be used as an important marker for early tumor diagnosis. In addition, circulating tumor cells also play an important role in tumor metastasis and recurrence. Therefore, it is of great significance to study the physiological mechanism of circulating tumor cells. However, the number of circulating tumor cells and their absolute concentration in human peripheral blood are very low, making the research on circulating tumor cells very difficult, so the sorting and capture of circulating tumor cells is an important research topic. [0003] At present, the commonly used cell sorti...

AI Technical Summary

Problems solved by technology

However, as an epithelial cell surface marker, EpCAM has different expression levels in different types of tumor cells, and its use is limited by the type of tumor cells.

In addition, the chemical modification steps for the substrate are cumbersome and time-consuming, which increases the success rate of uncertainty interference experiments

In addition, by manipulating the standing wave of the surface acoustic wave, the acoustic radiation force is used to directly manipulate the tumor cells for sorting and capture; the method of dielectrophoresis is used to sort the cells by using the difference in force of the dielectric particles in the uneven electric field. and capture, but these methods inevitably have adverse effects on cell viability

[0004] At present, the existing method of using microfluidic chips to capture tumor cells, first of all, the effect of capturing tumor cells is not very good. In addition, after capturing tumor cells, they lack the ability to analyze the cells on the chip, and they need to be transferred to centrifuge tubes before subsequent experiments can be performed. , the transfer process is not easy to do and will cause a large amount of cell loss, so there are insurmountable defects

Images