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Method for processing tobacco waste or organic fluorine wastewater

A tobacco waste and wastewater technology, applied in the field of applied microorganisms, can solve the problems of environmental and human impact, organic fluorine teratogenicity, difficult wastewater treatment, etc., to reduce costs and maintenance costs, reduce COD value, and less dosing times Effect

Active Publication Date: 2013-11-06
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, while the fluorine chemical industry is booming, the industrial wastewater it produces has also brought us great harm. Organic fluorine has teratogenic, mutagenic and carcinogenic properties. If it exists in the environment for a long time, it will definitely affect the environment and the environment. The human body makes an impact, so we have to deal with it
[0005] For a long time, for high-concentration fluorine-containing organic wastewater, methods such as chemical treatment and incineration are generally used first, and subsequent treatment is carried out after the concentration is reduced. However, these methods cannot fundamentally solve the problem, and the treatment cost is relatively high.
After the wastewater from the organic fluorine processing plant is treated by the anaerobic process in the early stage, it will produce wastewater containing 4-fluoroaniline that enters the aerobic stage. This type of wastewater is usually difficult to treat

Method used

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  • Method for processing tobacco waste or organic fluorine wastewater
  • Method for processing tobacco waste or organic fluorine wastewater
  • Method for processing tobacco waste or organic fluorine wastewater

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Example 1 Treatment of Tobacco Waste Using Arthrobacter TW and Signal Molecule Preparations Facilitating its Colonization

[0038] (1) Screening and cultivation of Arthrobacter TW:

[0039] Tobacco waste from Hangzhou Liqun Cigarette Factory was taken and continuously cultured for 14 days to enrich nicotine-degrading bacteria; the enriched culture was diluted and inoculated on nicotine inorganic salt medium for culture; colonies were picked and continued to be diluted and cultured. until a single colony was picked.

[0040] After identification, the single colony was Arthrobacter sp. (Acinetobacter sp.), named Arthrobacter TW. Arthrobacter TW has been preserved. Deposit unit: General Microbiology Center of China Microbiological Culture Collection Management Committee, preservation date: October 2011, preservation number: CGMCC No.7.47. The strain is a Gram-negative bacterium with a size of (0.5-0.7×1.6-2.0 μm), and the colony of Arthrobacter TW is white and moist roun...

Embodiment 2

[0059] Example 2 Using Arthrobacter TW and signal molecule preparations conducive to its colonization to treat organic fluorine processing wastewater

[0060] (1) Culture of Arthrobacter TW:

[0061] The Arthrobacter TW obtained by screening in Example 1 is inoculated into the inorganic salt medium whose 4-fluoroaniline concentration is 1.0g / L, and the medium formula is: K 2 HPO 4 0.2g / L, KH 2 PO 4 0.8g / L, NaMoO 4 ·H 2 O 0.0033g / L, MgSO 4 0.2g / L, CaSO 4 2H 2 O 0.1g / L, FeSO 4 ·7H 2 O0.005g / L, 4-fluoroaniline 1g / L, pH8.0; cultivated in a shaker at 130rpm and 30°C for 32h.

[0062] (2) Preparation of signal molecule preparations that are beneficial to TW colonization: the signal molecule preparations are composed of N-3 oxo-hexanoyl-homoserine lactone (3-oxo-C 6 -HSL) and N-hexanoyl-homoserine lactone (C 6 -HSL), the concentrations of the mother liquors are 8mmol / L and 4mmol / L respectively, the mixing ratio of the two is 1:1, and the total volume is 10mL. The functio...

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Abstract

The invention discloses a method for processing tobacco waste or organic fluorine wastewater. The method for processing tobacco waste comprises the following steps: adding tobacco waste into water and uniformly mixing to obtain a mixed liquor, adding activated sludge, an Arthrobacte TW seed liquid and a signal molecule preparation, and carrying out aeration treatment for 18-48h. The method for processing organic fluorine wastewater comprises the following steps: adding activated sludge, an Arthrobacte TW seed liquid and a signal molecule preparation into organic fluorine wastewater, and carrying out aeration treatment for 24-72h, wherein the collection number of the Arthrobacte TW is CGMCC No.7.47; and the signal molecule preparation is prepared by mixing an N-3oxo-caproyl-homoserinelactone solution and an N-caproyl-homoserinelactone solution. According to the method, adding frequency is low. The method is economical and environmentally friendly, can be adopted to preferentially, rapidly and efficiently degrade nicotine in tobacco or 4-fluoroaniline in organic fluorine wastewater so as to reduce the harm, and is of great significance for environmental protection.

Description

technical field [0001] The invention relates to the technical field of applied microorganisms, in particular to a method for treating tobacco waste or organic fluorine wastewater. Background technique [0002] Nicotine is the main alkaloid in tobacco leaves, which has a significant toxic effect on the human body. my country's tobacco industry produces about 1.3-1.7 million tons of tobacco waste every year, and the average content of nicotine in dry waste is as high as 18g / kg, which is 36 times higher than the European Union's toxic and harmful tobacco waste control standards for nicotine content; it produces tens of millions of tobacco processing wastewater tons, and its nicotine content is also as high as 1.0g / L, and these wastes are all considered as "poisonous and dangerous wastes". Ecotoxicology tests have shown that nicotine is soluble in water and various organic solvents, can pass through complex biofilm systems such as the blood-brain barrier, and produce toxicity to...

Claims

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Application Information

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IPC IPC(8): B09B3/00C02F3/12C02F3/34C02F101/36
CPCY02W10/10
Inventor 汪美贞郑昕王珏华许俊杰王硕何虹蓁闵航沈东升
Owner ZHEJIANG GONGSHANG UNIVERSITY
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