Method for preparing purified foot-and-mouth disease vaccine

Abstract

The invention discloses a method for preparing a purified foot-and-mouth disease vaccine, a serum or animal-derived ingredient free culture medium and an application of the serum or animal-derived ingredient free culture medium to the preparation of the foot-and-mouth disease vaccine, belonging to the filed of biotechnology. The method for preparing the purified foot-and-mouth disease vaccine comprises the following steps of: culturing a foot-and-mouth disease virus by using the serum or animal-derived ingredient free culture medium, purifying an obtained virus solution to obtain a purified antigen, subjecting a cell strain BHK-21 or BSR to the multiple-generation acclimatization culture and the suspension culture by 300L of a microcarrier through the serum-free culture medium, inoculating the cell strain BHK-21 or BSR against the foot-and-mouth disease vaccine, stirring at the rotating speed of 30-50rpm, microfiltrating, ultrafiltrating, concentrating 50-200 times, carrying out chromatography with a Sephawse6FF molecular sieve or density gradient zonal centrifugation with a continuous flow, and inactivating with beta-propiolactone to obtain the serotype univalent or multivalent vaccine for cattle, sheep and pigs.

Description

technical field [0001] The invention relates to a preparation method of a purified foot-and-mouth disease vaccine, and also relates to a serum-free or animal-derived component-free culture medium and its application in preparing the foot-and-mouth disease vaccine. Belongs to the field of biotechnology. Background technique [0002] Foot-and-mouth disease is an acute, highly contagious, febrile infectious disease of artiodactyls caused by Foot-and-mouth disease virus (FMDV), known for its rapid spread and high infection rate. Once the disease breaks out, it will cause huge economic losses to the affected country. Countries all over the world attach great importance to the research of this disease, and the International Veterinary Bureau ranks it as the first class A infectious disease. Foot-and-mouth disease is a member of the genus Foot-and-Mouth Virus in the family Picornaviridae, and has seven serotypes: A, O, C, Asia I, SA T1, SA T2 and SA T3. At present, the prevention...

AI Technical Summary

Problems solved by technology

[0004] There are two main problems in the existing inactivated foot-and-mouth disease vaccines: 1. The immune protection is not stable, mainly manifested in: after immunization, neutralizing antibodies cannot be detected or the antibody level is very low, or the immunization period is short, only 2 to 3 2. Large immune side effects, mainly: the downstream production process is still the traditional method without purification treatment, there are some impurities and allergic substances in the culture medium and host cells in the vaccine, and the effective antigen content is low

This has a great impact on the prevention and control of foot-and-mouth disease

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