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Application of berberine in preparation of weight-loss drug

A technology of berberine and medicine, which is applied in the field of pharmaceutical preparation, can solve problems such as no research, and achieve a significant inhibitory effect

Inactive Publication Date: 2013-10-23
RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Whether the autophagy pathway is involved in the regulation of berberine on lipid metabolism, there is no relevant research

Method used

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  • Application of berberine in preparation of weight-loss drug
  • Application of berberine in preparation of weight-loss drug
  • Application of berberine in preparation of weight-loss drug

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Experimental program
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Embodiment Construction

[0024] Below in conjunction with embodiment, the present invention will be further described:

[0025] Berberine (Berberine) used in the experiment was purchased from Shanghai Drug Administration and Inspection Institute; mesoderm pluripotent stem cell line C3H10T1 / 2 cells were purchased from ATCC (American Type Culture Collection) in the United States.

[0026] 1. Cell culture and induced differentiation

[0027] (1) Cultivation and passage of C3H10T1 / 2 mesoderm pluripotent embryonic stem cell line:

[0028] C3H10T1 / 2 cells were routinely cultured in 10% FBS / DMEM medium in 5% CO 2 , Incubate in an incubator at 37°C. After the cells reach 80%-90% confluence, suck out the culture medium with a pipette in the ultra-clean bench, add 5mL of 0.25% trypsin to digest, the cells shrink into a round shape under the microscope, add normal 10% FBS / DMEM culture medium to stop the trypsin digestion reaction, blow the residual cells on the bottle wall repeatedly with a pipette to make the...

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Abstract

The invention belongs to the field of pharmaceutical preparation, and particularly provides an application of berberine in preparation of a medicine used for inhibiting autophagy of adipocyte and a medicine used for treating obesity. According to the application, C3H10T1 / 2 cell is cultured and induced to be differentiated into mature adipocyte, then the test for the impact of berberine to the mature C3H10T1 / 2 adipocyte autophagy marker protein LC3 and an autophagy substrate P62, and the test for detecting the C3H10T1 / 2 flat autophagy flux variation through chloroquine serving as a lysosomal inhibitor are performed, and the variation of the quantity of autophagosome can be observed through a transmission electron microscope; the results of various tests show that the berberine can effectively inhibit the autophagy of C3H10T1 / 2 adipocyte and provides theoretical basis for clinically reasonably applying the berberine to the treatment of obese patients; in addition, the berberine also can be applied to the preparation of the medicine for inhibiting the autophagy of adipocyte.

Description

technical field [0001] The invention belongs to the field of medicine preparation, and particularly relates to the application of berberine in the preparation of drugs for inhibiting fat cell autophagy and the preparation of drugs for treating obesity. Background technique [0002] With the improvement of economic level, the change of people's diet structure and the reduction of exercise, the prevalence of obesity has risen rapidly. It is estimated that there are about 1 billion overweight or obese people in the world. Obesity, as one of the main components of metabolic syndrome, is closely related to various diseases such as type 2 diabetes, dyslipidemia, hypertension, coronary heart disease, and seriously endangers human health. [0003] In recent years, studies have found that autophagy plays an important role in the lipid metabolism of liver and adipose tissue. Autophagy is a process that engulfs its own cytoplasmic proteins or organelles and encapsulates them into vesic...

Claims

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Application Information

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IPC IPC(8): A61K31/4375A61P3/04
Inventor 宁光张晓燕杨颖
Owner RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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