Method for transforming sterile line of Chinese kale by utilizing vernalization and generation adding

Abstract

The invention relates to a method for transforming a sterile line of Chinese kale by utilizing vernalization and generation adding. The method comprises the following steps of: carrying out hybridization by taking a male sterile line of broccoli cytoplast as a female parent and a selfing line of the Chinese kale as a male parent, so as to obtain hybridized seeds; after the hybridized seeds are planted in summer, carrying out vernalization on plants, and meanwhile, selecting the sterile plants by utilizing pollen microscopic examination or single-plant bagging selfing; and taking the selfing line of the Chinese kale as a recurrent parent, carrying out 3-5-generation backcross with the recurrent parent and the sterile plants, so as to obtain the male sterile line of Chinese kale cytoplast. According to the method, the sterile line of the Chinese kale is transformed by utilizing the male sterile line of broccoli cytoplast, biological characters of the sterile line of the Chinese kale are stable, stamens degenerate, petals and pistils develop normally, nectaries develop well, and seed pods develop normally; the sterile line can be subjected to generation adding propagation under a normal growth condition of the plants, the transformation process is accelerated, the procedures are simplified, the cost is saved, and the purity of the Chinese kale can be improved.

Description

technical field [0001] The invention relates to a method for transferring male sterile lines of kale, in particular to a method for rapidly transferring male sterile lines of Chinese kale by means of vernalization, and belongs to the technical field of plant cultivation. Background technique [0002] Kale (Brassica oleracea var 1 alboglabra Bailey) is a Brassica genus in the Brassicaceae family, a cabbage vegetable that is eaten with young leaves and flower sprouts. According to the ancient documents of our country, kale originated in southern China. As early as the Northern and Southern Dynasties in the 5th and 6th centuries AD, the vegetable-eating loose-leaf cabbage (Brassica oleracea L1) was introduced to China from its original area through Central Asia, and it was called Xitulan at that time. Afterwards, through gene mutation and long-term artificial breeding in Guangdong area under subtropical climate conditions, kale with young leaves and flowering shoots was finall...

AI Technical Summary

Problems solved by technology

Due to the small organs of kale flowers, it takes a lot of manpower and time to reproduce the parents when using self-incompatible lines to prepare hybrids. The cost is very high, and the promotion area is limited by the number of controlled species, and the long-term continuous self-trading of the parents generates vitality. degeneration, so that some excellent characters are lost, and the hybridization rate of hybrids is difficult to reach 100%. The use of male sterile lines for seed production can overcome the above defects, so breeders actively carry out the selection of Chinese kale male sterile lines

As far as the applicant knows, one of the key points of male sterile line breeding is to determine the source of male sterility. There are two sources of male sterility: nuclear male sterility and cytoplasmic male sterility. The source of nuclear male sterility is divided into There are two types of recessive and dominant. Among them, the recessive nuclear male sterile line is difficult to find because of the maintainer line, and the seeds of the sterile line cannot be mass-produced for seed production. The dominant nuclear male sterile line is used for seed production and transformation The procedures are complicated, and the sterile plants used to reproduce the male sterile lines need to be multiplied by tissue culture, and the sterility is difficult to maintain. When the dominant nuclear male sterile lines are pollinated by normal fertile plants, their offspring will be counted as 1 :1 ratio can separate dominant sterile plants and recessive fertile plants, and pass this method from generation to generation, which is not easy to promote in agricultural production

Kale cytoplasmic male sterile materials can choose cabbage vegetable cytoplasmic male sterile materials, there are two types of radish cytoplasm and black mustard cytoplasm, the black mustard cytoplasmic male sterile has been proven to be unusable, and some domestic units have collected Most of the male sterile lines of radish cytoplasmic cabbage are difficult to use because of abnormal plant growth and flowering and fruiting traits

The cytoplasmic male sterile materials of cabbage collected so far, when used as the sterile source of Chinese cabbage male sterile lines, in the actual transgenic process, the number of backcross generations is longer, the traits of flowering and fruiting are poor, the uniformity is poor, and the offspring deformity rate high, cannot be used directly

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