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Test sample preparation method of tobacco pectin content detection

A sample preparation and tobacco technology, which is applied in the field of sample preparation for the detection of tobacco pectin content, can solve problems such as interference in color development, interference in measurement results, and cumbersome procedures

Inactive Publication Date: 2013-10-02
CHINA TOBACCO GUANGDONG IND
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the problem with spectrophotometry is that some small molecular sugars will interfere with color development, and the pigments in the solution will also interfere with the absorbance measurement results.
However, the existing enzymatic hydrolysis-continuous flow method for the determination of pectin has some problems due to improper pre-treatment settings: First, there are some natural neutral sugar branched chains in pectin, which cannot be eliminated during the sugar removal process. Extracted, but will be released when the pectin is hydrolyzed, thereby causing serious interference to the test results of the existing pectin content continuous flow analysis method; secondly, using the enzymatic hydrolysis method to hydrolyze the sample pectin, not only the procedure is cumbersome, but the pretreatment cycle long, seriously affects work efficiency, and its reliability is poor; in addition, some pectin components are water-soluble, and 80% ethanol solution is used to remove sugar, especially acetic acid solution in pretreatment, which is likely to cause water-soluble pectin the loss of; so the processing method in the prior art, the prepared sample to be tested can make the detection result not accurate enough when measuring with a continuous flow measuring instrument, and the operation is complicated and the cycle is long

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  • Test sample preparation method of tobacco pectin content detection
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  • Test sample preparation method of tobacco pectin content detection

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preparation example Construction

[0036] The sample preparation method of tobacco pectin content detection of the present invention comprises the following steps:

[0037] 1) Add the tobacco sample into the pretreatment liquid, heat and reflux in a water bath at 90°C to 100°C for 10 to 30 minutes, the pretreatment liquid has a hydrogen ion concentration of 0.005mol / L to 0.015mol / L and an alcohol volume concentration of 75% to 85% acid alcohol solution;

[0038] 2) Then use glass fiber filter paper for initial filtration, and rinse the filter residue from the initial filtration with acid alcohol solution;

[0039] 3) Add the rinsed filter residue to the extract solution with a hydrogen ion concentration of 0.75mol / L-1.25mol / L, and heat to reflux in a water bath at 80°C-100°C for 1.51h-2.53h;

[0040] 4) Then carry out secondary filtration, rinse the filter residue after secondary filtration with acid solution for several times, then combine the filtrate after secondary filtration with the rinsing solution and ...

Embodiment 1

[0044] 1) Add the powdered tobacco sample into the pretreatment solution, and heat and reflux in a water bath at 100°C for 10 minutes;

[0045] 2) Then use glass fiber filter paper for primary filtration, and rinse the filter residue of the primary filtration with acid alcohol solution for several times;

[0046] 3) Add the rinsed filter residue to the extract solution with a hydrogen ion concentration of 1.25mol / L, and heat to reflux in a water bath at 100°C for 1.5h;

[0047] 4) Then use glass fiber filter paper for secondary filtration, rinse the filter residue after secondary filtration with acid solution for several times, then combine the filtrate and rinse solution, and constant volume to obtain the sample to be tested.

[0048] Wherein, the pretreatment solution is an acid-alcohol solution, the hydrogen ion concentration of the acid-alcohol solution is 0.005mol / L, and the alcohol concentration of the acid-alcohol solution is 75% in volume percentage.

Embodiment 2

[0050] 1) Add the powdered tobacco sample into the pretreatment solution, and heat and reflux in a water bath at 90°C for 20 minutes;

[0051] 2) Then use glass fiber filter paper for primary filtration, and rinse the filter residue of the primary filtration with acid alcohol solution for several times;

[0052] 3) Add the rinsed filter residue to the extract solution with a hydrogen ion concentration of 0.9mol / L, and heat to reflux in a water bath at 90°C for 2.5h;

[0053] 4) Then use glass fiber paper for secondary filtration, rinse the filter residue after secondary filtration with acid solution for several times, then combine the filtrate and rinse solution, and constant volume to obtain the sample to be tested.

[0054] Wherein, the pretreatment solution is an acid alcohol solution, the hydrogen ion concentration of the acid alcohol solution is 0.01mol / L, and the alcohol concentration of the acid alcohol solution is 80% in volume percentage.

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Abstract

The invention discloses a test sample preparation method of tobacco pectin content detection. The test sample preparation method comprises the following steps: 1) adding a tobacco sample to a pre-treatment solution, and carrying out heating reflux for 10-30 min in a 90-100 DEG C water bath, wherein the pre-treatment solution is an acid alcohol solution with a hydrogen ion concentration of 0.005-0.015 mol / L and an alcohol volume concentration of 75-85%; 2) adopting glass fiber filtration paper to carry out primary filtration, and rinsing filter residue of the primary filtration by using the acid alcohol solution; 3) adding the rinsed filter residue to an extraction liquid with a hydrogen ion concentration of 0.75-1.25 mol / L, and carrying out heating reflux for 1.5-2.5 h in a 90-100 DEG C water bath; and 4) adopting glass fiber filtration paper to carry out secondary filtration, rinsing filter residue of the secondary filtration a plurality of times by using an acid solution, mixing the filtrate of the secondary filtration and the rinsing liquid, and carrying out volume metering to obtain the test sample to be detected. With the test sample prepared by using the preparation method, detection result accuracy can be improved in the subsequent detection, the operation process is short, and efficiency is high.

Description

technical field [0001] The invention relates to the measurement of pectin content in tobacco, in particular to a sample preparation method for detection of tobacco pectin content. Background technique [0002] Pectin is found in the cell wall and inner layer of plants and is an important component of plant cells. Pectin is a derivative of carbohydrates, mainly linear galacturonic acid, and the basic component of the main chain molecule is D-galacturonic acid, which is connected by α-1,4-glucosidic bonds to become a polymer compound. In tobacco leaves with different sources and processing techniques, the pectin content has a large difference, and the difference is about 5%-20% in weight percentage. The pectin content in tobacco not only affects the physical and chemical properties of tobacco leaves, but also contributes to the aroma of cigarettes to a certain extent, but if the proportion is too high, it will also have a negative impact on tobacco smoking and smoking safety....

Claims

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Application Information

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IPC IPC(8): G01N1/28
Inventor 孔浩辉程志颖
Owner CHINA TOBACCO GUANGDONG IND
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