Application of beta-carotene in preparation of pig sperm cryoprotectant
A technology of cryoprotectant and carotene, which is applied in the biological field, can solve the problems of sperm cell membrane damage, pig sperm fertilization ability decline, etc., and achieve the effect of being easy to popularize, improving pig sperm viability, and maintaining fertilization vitality
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0027] Accurately measure glucose: 1.1g, citric acid: 1.48g, Tris: 2.42g, penicillin sodium: 0.06g, streptomycin sulfate: 0.1g Dissolve in 100ml double distilled water, adjust pH to 6.21, osmotic pressure 286mosm / L, Formulated into frozen base fluid;
[0028] Accurately measure 80ml of conventional frozen base solution, add 20ml of fresh egg yolk and 1mg to 1.5mg of β-carotene, and mix well to prepare Ⅰ solution;
[0029] Take 97ml of solution I and add 3ml of glycerin to prepare 100ml of solution II, adjust the pH of solution II to 6.0-7.5, and the osmotic pressure to 286mosm / L, filter and sterilize, cool to room temperature, and put it in a refrigerator at 4°C for later use.
Embodiment 2
[0031] Accurately measure glucose: 1.1g, citric acid: 1.48g, Tris: 2.42g, penicillin sodium: 0.06g, streptomycin sulfate: 0.1g Dissolve in 100ml double distilled water, adjust pH to 6.21, osmotic pressure 286mosm / L, Formulated into frozen base fluid;
[0032] Accurately measure 80ml of frozen base solution, add 20ml of fresh egg yolk and 1.5mg to 2.0mg of β-carotene, and mix well to prepare liquid I;
[0033] Take 97ml of liquid I and add 3ml of glycerin to prepare 100ml of liquid II, adjust the pH of liquid II to 6.0-7.5, and the osmotic pressure to 286mosm / L, filter and sterilize, cool to room temperature, and put it in a refrigerator at 4°C for later use.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com