Micro-fluidic chip fluorescence excitation device, micro-fluidic chip and preparation method thereof

Abstract

The invention discloses a micro-fluidic chip fluorescence excitation device comprising nano optical fibers in a smooth cone shape and nanowires connected to the cone tip of the nano optical fibers. The nano fibers are made of high-molecular polymers. In this way, in the micro-fluidic chip fluorescence excitation device of the invention, the cone tip of the nano optical fibers is stretched into a micro channel of the micro-fluidic chip. The cone tip is corresponding to a specified point to be detected. The nanowires at the cone tip are melted so that the nano optical fibers can be fixed in the micro channel. Fluorescent excitation light irradiates and enters in through a thick end of the nano optical fibers. The excitation light is introduced by the nano optical fibers to the micro channel and concentrated in the cone tip for irradiation outwards. Fixed-point fluorescent excitation is conducted at the specified points to be detected in order to effectively avoid the interference of excitation to fluorescence signals of a sample and increase sensitivity and reliability of detection; the invention also discloses a micro-fluidic chip and a preparation method thereof. The micro-fluidic chip can not only increase the sensitivity and reliability of the detection, but also can increase the integration level of the fluorescence excitation device and the chip; the preparation method thereof is simple, direct and efficient.

Description

technical field [0001] The invention relates to a microfluidic chip detection technology using a laser to excite fluorescence of a substance in the fields of biology and chemistry, and in particular to a microfluidic chip fluorescence excitation device, a microfluidic chip and a preparation method thereof. Background technique [0002] It is an important biochemical detection method to use laser to excite the fluorescence of the detection substance and analyze the composition and concentration of the sample. Microfluidic chips can integrate basic operating units such as sample preparation, biochemical reactions, separation, and detection in the fields of biology and chemistry on centimeter-scale chips. [0003] In recent years, in the field of microfluidics, with the continuous improvement of the requirements of microfluidics technology, the size of microchannels of microfluidics chips has been gradually reduced. However, for the microfluidic chip whose microchannel size is...

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Problems solved by technology

However, for the microfluidic chip whose microchannel size is further reduced, the traditional method of direct laser irradiation or the method of embedding optical fibers in the chip will interfere with the large size of the fluorescence excitation device and the large illumination surface. The fluorescence signal of the sample in the microchannel affects the detection sensitivity and reliability of the sample; in addition, the direct irradiation excitation method separates the fluorescence excitation device and the microfluidic chip into two independent experimental units, which greatly reduces the overall application cost. The integration level of the detection system of the microfluidic chip

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