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Lactococcus garvieae and biological feed-additive prepared therefrom

A feed additive, Lactococcus gasseri technology, applied in the field of microorganisms, can solve the problems of high price, high dosage, complex components, etc., and achieve the effects of reducing feed consumption, promoting growth and good stability

Inactive Publication Date: 2014-12-17
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The obvious disadvantages of compound bacterial agents are that the price is high, the ingredients are too complicated, and the amount of addition is also high. Although it is effective, it cannot bring rich economic income to the farmers.

Method used

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  • Lactococcus garvieae and biological feed-additive prepared therefrom
  • Lactococcus garvieae and biological feed-additive prepared therefrom
  • Lactococcus garvieae and biological feed-additive prepared therefrom

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: each culture characteristic of bacterial strain SYP-B-301 of the present invention

[0018] After the Lactococcus gasseri SYP-B-301 strain was cultured on the medium at 25-34° C. for 24 hours, the colony morphology was observed, and the plate showed a transparent round colony with a smooth surface and a colony diameter of 1-2 mm. The strain is red after Gram staining, and under a microscope, it is found that the bacteria is a regular spherical shape with a diameter of 0.5-0.8um, no spores, flagella, capsules, no movement, single, paired or short chains.

[0019] Genomic DNA of strain SPY-B-301 was extracted, and PCR was performed with Primer (S): 5'AGA GTT TGA TCC TGG CTC AG3'; and Primer (A): 5'AAG GAG GTG ATC CAG CCG CA3' as primers, Amplify the 16s rDNA gene of the strain. After purification, gene sequencing was carried out, and the measured sequence was compared with the rDNA homologous sequence in Genbank, 15 strains with higher homology were selecte...

Embodiment 2

[0022] Embodiment 2: take mouse as model animal, research the influence of SYP-B-301 feed additive on mouse growth performance

[0023] Experimental animals: 20 Kunming male mice (about four weeks old) of 18-22 g were selected, and the test period was 15 days. Divide into control group and experimental group, each group has 10 experimental mice, and repeat the experiment twice.

[0024] Weigh every day from the beginning of the test, take each repetition as a unit, calculate the average daily weight gain of each group, and accurately record the feed intake and the amount of leftover material, observe the mental state of the mice in each group, the test period is 15d, calculate the average daily weight gain Weight gain, and accurately record the feed intake and leftover feed amount, calculate the average daily feed intake and feed conversion ratio of each group, and record the mortality rate and diarrhea rate. The experimental results are shown in Table 2.

[0025] Animal exp...

Embodiment 3

[0039] Embodiment 3: take weaned piglet as model animal, research the influence of SYP-B-301 feed additive on growth performance of weaned piglet

[0040] Select 28-day-old weaned piglets and randomly divide them into 2 treatments according to litter source, body weight, and sex. Each treatment has 6 replicates, and each replicate has 6-8 pigs. The male-to-female ratio is balanced. It started at about 56 days old and lasted for 28 days, ending at 56 days old.

[0041] Piglets were weighed on an empty stomach at 8:00 am at 28 days, 42 days and 56 days respectively, and the feed consumption at this stage was settled on the day of weighing. The average daily feed intake, average daily gain, feed-to-weight ratio, mortality rate and diarrhea rate of piglets at each stage were calculated.

[0042] The weight of the piglets was weighed at the beginning and end of the test, carried out on an empty stomach in the morning, the feeding trough of the piglets was cleaned up 12 hours before ...

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Abstract

The invention relates to the field of microbial technology, and relates to a strain of Lactococcus garvieae and a feed additive produced therefrom. The feed additive is a solid microbial inoculum prepared from a liquid microbial inoculum through addition of proper nutrient substances, wherein the liquid microbial inoculum is prepared from Lactococcus garvieae SYP-B-301 through fermentation culture in a fermentation cylinder. The feed additive has a wide range of applications in animal husbandry and poultry raising such as pig raising, chicken raising, cattle raising and the like. The feed additive has a plurality of functions, through use of the feed additive, animal immunity after eating can be improved, animal diarrhoea rate and death rate can be reduced, the use of antibiotic drugs can be reduced, drug residues can be eliminated, production efficiency can be obviously improved, the raising cycle can be shortened, and feed can be effectively saved, so that income of farmers can be increased.

Description

Technical field: [0001] The invention relates to the technical field of microbes, and relates to a strain of Lactococcus gasseri and a feed additive prepared by the bacteria. Background technique: [0002] Foreign probiotic preparations were first seen in Japan. The United States began to use feed microorganisms in the 1970s. The research on probiotics in my country began in the 1980s. It started relatively late, but developed rapidly. At present, it mainly focuses on the research of lactic acid bacteria and spores Bacteria predominate. In recent years, the application of probiotic preparations in poultry farming has played a significant role, and has gradually been affirmed by the majority of farmers. [0003] In recent years, the disadvantages of using antibiotics as feed additives have become more and more obvious. It can no longer effectively prevent and control animal diseases, and the drug residues caused have a great impact on both animals and humans. As antibiotics ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16C12R1/46
Inventor 张怡轩
Owner SHENYANG PHARMA UNIVERSITY
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