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Preparation method of active polysaccharide of male agaric mycelium

A technology of Phellinus mycelium and active polysaccharides, applied in botany equipment and methods, allergic diseases, horticulture, etc., can solve the problems of high cultivation cost, impact on application, and low purity of active polysaccharides, and achieve simple and effective technology Immunocompetent, easily scalable effects

Inactive Publication Date: 2013-09-25
SHANGHAI ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] However, the existing Phellinus Phellinus has high cultivation costs and low purity of active polysaccharides, which affect its further application in pharmacy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Preparation of bag culture medium

[0018] Configure 59% sawdust, 20% bran, and 1% gypsum, mix with water, and keep the water content at about 65%. Use polypropylene bags (17cm x 33cm x 0.0005cm) to fill the material, each bag contains 1kg of wood chip medium wet material, and autoclave at 121°C for 120min.

[0019] (2) Cultivation of mother species, original species, cultivated species and mycelium

[0020] Phellinus bacterium is grown on the slope of PDA, and the growth cycle is one week, which is the mother species of Phellinus.

[0021] Take the mixed sawdust culture medium and put it into a 500ml Erlenmeyer flask to one-half, make about 5 bottles (1 bottle of mother seed can make 16 bags of original seed), autoclave for 120min, and after cooling, connect Phellinus mother seed to the culture medium Above, the original species of Phellinus Phellinus were obtained by culturing in a constant temperature incubator in the dark for 10 days at 28°C.

[0022] Turn 5 ...

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PUM

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Abstract

The invention discloses a preparation method of active polysaccharide of male agaric mycelium. On the one hand, cheap substrates, such as sawdust and bran, are fermented in the solid state; the cost is low; the technology is simple; the process can be carried out by general farmers; the preparation method is easy to popularize; one the other hand, the method disclosed by the invention is capable of effectively increasing the purity of crude polysaccharide of male agaric mycelium; the prepared crude polysaccharide of male agaric mycelium has good immunological competence.

Description

technical field [0001] The invention relates to the field of edible fungus cultivation and processing, in particular to a method for preparing active polysaccharides from Phellinus mycelium. Background technique [0002] Phellinus baumii Pilat, commonly known as Phellinus, is a perennial, yellow-brown precious medicinal fungus, known as "forest gold". Because of its remarkable anti-tumor effect, it has become a research hotspot at home and abroad, and it is popular in Japan and South Korea, and its best-selling is not bad. [0003] As a rare medicinal fungus, the main active ingredient of Phellinus Phellinus is polysaccharides. At present, the research on Phellinus Phellinus is mostly focused on the extraction of polysaccharides and their anticancer effects. A large number of experiments have shown that Phellinus mycelium and fruiting body extracts have good anti-tumor effects. Because the growth cycle of the fruiting body of Phellinus Phellinus is relatively long, the pri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P37/04A01G1/04
Inventor 杨焱李婷婷周峰唐传红张劲松刘艳芳邵倩李正鹏周帅
Owner SHANGHAI ACAD OF AGRI SCI
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