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Method for improving perennial ryegrass callus regeneration rate

A technology of callus and ryegrass, applied in the field of plant tissue culture, to achieve the effect of shortening the transformation cycle, optimizing the genetic transformation program, and high transformation rate

Inactive Publication Date: 2013-09-25
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention is mainly aimed at problems such as difficult regeneration of perennial ryegrass callus, low regeneration rate and restriction by genotype, and provides a method for improving perennial ryegrass callus regeneration rate method

Method used

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  • Method for improving perennial ryegrass callus regeneration rate
  • Method for improving perennial ryegrass callus regeneration rate
  • Method for improving perennial ryegrass callus regeneration rate

Examples

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Effect test

example 1

[0047] Take 5g of ryegrass lark seeds and put them into a culture bottle, soak them in tap water for 16-24 hours, pour out the seeds floating on the water surface together with the water, and keep the plump seeds in the lower layer for disinfection. Stir the plump seeds with sulfuric acid with a volume fraction of 50% for 10 minutes to remove the shells, shake continuously during the period, pour off the sulfuric acid, and rinse with tap water for 3-4 hours. Put the rinsed seeds into a mortar and grind lightly to remove the shells, pour some water, the ground shells float on the surface of the water and will be poured out with the water. The treated seeds were incubated in a 56°C water bath for 15 minutes at a constant temperature to kill endophytes closely related to ryegrass. On the ultra-clean workbench, disinfect with 75% alcohol for 1 min, rinse with sterile water for 3 times, then disinfect with 0.1% mercuric chloride solution for 10 min, rinse with sterile water for 6-8...

example 2

[0059] Take 5g of ryegrass 'gold medal' seeds and put them into a culture bottle, soak them in tap water for 16-24 hours, pour out the seeds floating on the water surface together with the water, and keep the plump seeds in the lower layer for disinfection. Stir the plump seeds with sulfuric acid with a volume fraction of 50% for 10 minutes to remove the shells, shake continuously during the period, pour off the sulfuric acid, and rinse with tap water for 3-4 hours. Put the rinsed seeds into a mortar and grind lightly to remove the shells, pour some water, the ground shells float on the surface of the water and will be poured out with the water. The treated seeds were incubated in a 56°C water bath for 15 minutes at a constant temperature to kill endophytes closely related to ryegrass. On the ultra-clean workbench, disinfect with 75% alcohol for 1 min, rinse with sterile water for 3 times, then disinfect with 0.1% mercuric chloride solution for 10 min, rinse with sterile water...

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Abstract

The invention discloses a method for improving the perennial ryegrass callus regeneration rate, and relates to the technical field of plant tissue culture. The method comprises the following steps of: (1) screening seeds; (2) treating the seeds; (3) disinfecting the seeds; (4) preparing a culture medium, comprising inducing of a culture medium, subculturing of the culture medium, differentiating of the culture medium, and maintaining of the culture medium for plants; (5) inducing callus; (6) subculturing; (7) differentiating the callus tissue; (8) maintaining and culturing plants of regenerated seedlings; and (9) inducing the callus tissue with a good regeneration performance. The method for improving the perennial ryegrass callus regeneration rate not only optimizes the genetic transformation program, greatly shortens the transformation cycle, guarantees higher transformation rate, but also provides a favorable acceptor material for the subsequent genetic transformation.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for improving the regeneration rate of perennial ryegrass callus. technical background : [0002] perennial ryegrass ( Lolium perenne L.) is a perennial herb belonging to the family Poaceae Lolium, and is an important cool-season pasture and lawn grass in my country. The perennial ryegrass has excellent grass quality, abundant leaves, tender stems and leaves, and good palatability. It is an excellent pasture grass for horses, cattle, sheep, and rabbits. It is also a good feed for fish. It has high yield and high nutritional value. It is a herb Excellent forage for multiple uses. At the same time, perennial ryegrass is a cool-season grass species, which grows quickly into a lawn and has a beautiful plant shape. It is often used as a pioneer grass species in gardens and scenic spots, mixed with bluegrass and other varieties, and can also be mixed with Ber...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 傅金民郭慧娟娄燕宏
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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