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Gamma-glutamoyl transferase detection reagent

A technology for detecting reagents and glutamyl groups, which is applied in the field of medical testing, can solve the problems of lack of testing professionals and insufficient facilities for fully automatic biochemical testing equipment, and achieve good sensitivity, low price, and easy operation

Active Publication Date: 2013-09-11
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] With the "Eleventh Five-Year" plan for the purchase and strengthening of basic equipment for tertiary and secondary hospitals, hospitals above the middle level have good hardware and software facilities, but grassroots medical institutions, especially health centers, clinics and other units are not fully automatic. Biochemical testing equipment and facilities are generally insufficient, and there is not a sufficient number of testing professionals. Therefore, the establishment of a POCT analysis system that is easy to operate, low in price, and real-time reporting is of great significance to the role of grassroots hospitals in disease prevention and diagnosis and treatment.

Method used

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  • Gamma-glutamoyl transferase detection reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Diluent:

[0041] Tris(hydroxymethyl)amino buffer (pH7.0) 0.1mol / L

[0042] TWEEN 80 5%

[0043] Proclin300 0.1%

[0044] Bilirubin oxidase 1KU / L

[0045] Vitamin C oxidase 1KU / L

[0046] Reagent:

[0047] Tris buffer (pH7.5) 0.15mol / L

[0048] Dipeptide 0.1mol / L

[0049] L-γ-glutamyl-3-hydroxy-4-nitroaniline 10mmol / L

[0050] Copper chloride 10mmol / L

[0051] Potassium Sorbate 0.1g / L

[0052] Bovine serum albumin 5g / L

Embodiment 2

[0054] Diluent:

[0055] Tris(hydroxymethyl)amino buffer (pH7.5) 0.5mol / L

[0056] TRITON X-100 5%

[0057] Methyl p-hydroxybenzoate 1%

[0058] Potassium ferrocyanide 1mol / L

[0059] Vitamin C oxidase 2KU / L

[0060] Reagent:

[0061] 3-morpholinopropanesulfonic acid buffer (pH7.0) 0.2mol / L

[0062] Dipeptide 0.2mol / L

[0063] L-γ-glutamyl-3-hydroxy-4-nitroaniline 20mmol / L

[0064] Boric acid 50 mmol / L

[0065] Proclin300 1g / L

[0066] Trehalose 10g / L

Embodiment 3

[0068] Diluent:

[0069] 3-morpholinopropanesulfonic acid buffer (pH6.5) 1.0mol / L

[0070] SPAN 20 10%

[0071] Sodium benzoate 1%

[0072] Potassium ferricyanide 10mol / L

[0073] Vitamin C oxidase 10KU / L

[0074] Reagent:

[0075] Piperazine-N,N-bis(2-hydroxyethanesulfonic acid) buffer (pH8.0) 1mol / L

[0076] Dipeptide 1mol / L

[0077] L-γ-glutamyl-3-hydroxy-4-nitroaniline 100mmol / L

[0078] DMSO 100 mmol / L

[0079] Proclin300 1g / L

[0080] Fatty alcohol polyoxyethylene ether 10g / L

[0081] The performance of the reagent obtained in Example 1 of the present invention will be described below in conjunction with the table.

[0082] 1. Precision

[0083] Table 1. Precision evaluation results

[0084]

[0085] 2. Linear

[0086] The high-concentration serum samples collected from the hospital are diluted into different concentrations of analytes for detection, and the linear results of γ-GGT are shown in figure 1 ..

[0087] 3. Methodological comparison test

[0088] Compare with the GGT result value mea...

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Abstract

The invention discloses a gamma-glutamoyl transferase detection reagent which comprises a diluent and a reaction reagent, wherein the diluent is a buffer liquid, a surfactant, a preservative, a debilirubin and a vitamin C oxidase, and the reaction reagent is a buffer liquid, a bi-glycoside peptide, L-gamma-glutamoyl-3-hydroxyl-4-nitroaniline, a primer protector, a preservative and a freeze-drying protective additive. The detection reagent disclosed by the invention has good sensitivity, accuracy, precision and linearity, and can completely satisfy the clinical examination requirement.

Description

Technical field [0001] The invention relates to the technical field of medical testing, in particular to a gamma-glutamyltransferase detection reagent used in a POCT analyzer. Background technique [0002] Liver disease is a common and extremely harmful disease. Its timely diagnosis and treatment play an important role in the prevention, diagnosis and treatment of the disease. γ-glutamyltransferase (γ-glutamyltransferase, γ-GGT) is widely distributed in the human body Among the tissues, the kidney is the largest, followed by the pancreas and liver. The γ-GGT in normal human serum mainly comes from the liver. It only slightly increases in acute hepatitis, chronic active hepatitis and cirrhosis decompensation, but when obstructed In the case of sexual jaundice, the reverse flow into the blood due to excretion disorders. In the case of primary liver cancer, the hypersynthesis in the liver can be significantly increased, even more than 10 times the normal. The γ-GGT of alcoholism is ...

Claims

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Application Information

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IPC IPC(8): C12Q1/48C12Q1/26G01N21/31
Inventor 邹炳德邹继华沃燕波张桂春周海滨
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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