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A preparation method for lyophilized powder of earthworm fibrinolytic enzymes

A technology of plasmin and freeze-dried powder, which is applied in the field of biomedicine, can solve the problems of decomposition and destruction of enzymes and inhibitors, difficult to enter lesion cells and tissues, etc., and achieves prolonging half-life, eliminating immunogenicity and good biocompatibility. Effect

Inactive Publication Date: 2013-09-11
GUANGZHOU RAINHOME PHARM&TECH CO LTD
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AI Technical Summary

Problems solved by technology

Currently, commercially available Thrombolysis Capsules, Dilong Capsules, and "9.12" Capsules are just earthworm freeze-dried powders, which are easily decomposed and damaged by enzymes and inhibitors in the body, and are not easy to enter the lesion cells and tissues. Obviously, further research is needed to solve the problem.

Method used

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  • A preparation method for lyophilized powder of earthworm fibrinolytic enzymes
  • A preparation method for lyophilized powder of earthworm fibrinolytic enzymes
  • A preparation method for lyophilized powder of earthworm fibrinolytic enzymes

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Embodiment 1

[0033] The preparation of embodiment 1 earthworm fibrinolytic enzyme freeze-dried powder:

[0034] Step 1, the preparation of earthworm meal:

[0035] Live earthworms are starved for one day, spitting out the silt naturally, washed, then dried and pulverized under ventilated conditions with a temperature between 20-35°C, and passed through a 100-200 mesh sieve to make earthworm meal.

[0036] Earthworm fibrinolytic enzyme mainly exists in the digestive tract of earthworms. Therefore, in order to ensure the integrity of earthworm function, it is not suitable to remove internal organs during processing. Moreover, the drying conditions must be mastered. If the temperature exceeds 70°C, the active ingredients will be inactivated and denatured; if the temperature is too low and the drying time is too long, it will be easily contaminated by microorganisms and deteriorate. There is self-dissolution phenomenon, therefore, in the actual implementation, select the ventilated condition ...

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Abstract

The present invention discloses a preparation method for lyophilized powder of earthworm fibrinolytic enzymes. The preparation method comprises the steps of the preparation of earthworm meal, the preparation of a crude enzyme solution, the purification of crude enzymes, the modification of the earthworm fibrinolytic enzymes, and the preparation of lyophilized powder of the earthworm fibrinolytic enzymes. According to the preparation method for lyophilized powder of earthworm fibrinolytic enzymes of the present invention, on the basis of isolated and purified pure enzymes, activated polyethylene glycol is employed for modifying the earthworm fibrinolytic enzymes, so that the earthworm fibrinolytic enzymes have no direct contact with body fluids and the free amino groups are cleared, thus extending the half-life and eliminating immunogenicity of the earthworm fibrinolytic enzymes in the body.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a preparation method of earthworm fibrinolytic enzyme freeze-dried powder. Background technique [0002] Thrombosis is a common and frequently-occurring disease that seriously endangers human health, with high mortality and disability. When thrombosis occurs, the most effective treatment is thrombolysis. In recent years, thrombolytic drugs with earthworm fibrinolytic enzymes (EFEs) as the main active ingredient have played an important role in clinical practice. For example, BAIOO earthworm fiber Lysocapsules, Puenfu Capsules, etc. Earthworm fibrinolytic enzyme (EFE) has been extracted from earthworms since Miyazaki Medical University reported in 1984 that it has been extensively studied by Chinese, Japanese and Korean scholars. It is now proved that earthworm fibrinolytic enzyme (EFE) not only has the plasmin activity of directly dissolving fibrin, but also has the activat...

Claims

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Application Information

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IPC IPC(8): C12N9/68
Inventor 车七石陈良艳李新霞姚海珍
Owner GUANGZHOU RAINHOME PHARM&TECH CO LTD
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