Use of GLP-1 (Glucagon-Like Peptide-1) for preparing medicines for preventing and treating macrovascular complications of type 2 diabetes
A technology for type 2 diabetes and macrovascular, which is applied in the field of GLP-1 for the preparation of drugs for preventing and treating macrovascular complications of type 2 diabetes, and can solve problems such as inability to effectively cure diabetes and cardiovascular complications.
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Embodiment 1
[0015] Example 1: Construction of endothelial cell injury model of type 2 diabetes
[0016] In this example, high glucose and high insulin are used to stimulate vascular endothelial cells, simulating the environment of high glucose and high insulin in type 2 diabetes, and constructing an endothelial cell injury model.
[0017] Experimental animals and cells:
[0018] Healthy adult male / female SD (Sprague-Dawley) rats were purchased from the Experimental Animal Center of Southern Medical University, and rat aortic endothelial cells were isolated from the thoracic aorta of healthy adult male / female SD rats.
[0019] experimental method:
[0020] Healthy adult male / female SD rats were killed by cervical dislocation, the rat thoracic aorta was separated, the fatty tissue around the blood vessels was peeled off, the intima was everted, the two ends of the blood vessels were ligated with a ligature, and collagenase type Ⅰ (2g / L ) digested at 37°C for 45 minutes, cut blood vessels ...
Embodiment 2
[0040] Example 2: Effect of GLP-1 on apoptosis of rat aortic endothelial cells induced by high glucose and high insulin
[0041] In this example, on the basis of the diabetic vascular cell injury model constructed in Example 1, the protective effect of GLP-1 on diabetic vascular endothelial cell injury is systematically studied, and its mechanism is revealed. The method provides a basis.
[0042] Experimental animals and cells:
[0043] Healthy adult male / female SD rats were purchased from the Experimental Animal Center of Southern Medical University, and rat aortic endothelial cells were isolated from the thoracic aorta of healthy adult male / female SD rats.
[0044] experimental method:
[0045] The GLP-1 used in this experiment is GLP-1 (7-36), which was purchased from Shanghai Tricaptide Biotechnology Co., Ltd.
[0046] Rat aortic endothelial cells were plated, and when the cells grew to 85% of the bottom of the dish, the cells were starved overnight with sugar-free and ...
Embodiment 3
[0074] Embodiment three: pharmaceutical preparation
[0075] Natural GLP-1 or GLP-1 analogs are used as active ingredients, formulated in pharmaceutically acceptable excipients, carriers or diluents, adjusted to the filling concentration, filtered and sterilized, depyrogenated, filled, and prepared Injection, and further can be made into freeze-dried powder injection. For the relevant process, reference can be made to the conventional pharmaceutical process in this field.
[0076] These pharmaceutical preparations can be clinically used for intravenous infusion or subcutaneous injection.
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