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Preparation method of 96-well enzyme label plate chloramphenicol molecularly imprinted polymer film

A technology of molecular imprinting and chloramphenicol, applied in the direction of analysis materials, measuring devices, instruments, etc., can solve the problem of no chloramphenicol molecular imprinting polymer film, etc., achieve strong resistance to harsh environments, easy control of reaction conditions, and low cost cheap effect

Inactive Publication Date: 2013-09-04
DALIAN OCEAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, so far there is no relevant report on the preparation of molecularly imprinted polymer membranes of chloramphenicol

Method used

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  • Preparation method of 96-well enzyme label plate chloramphenicol molecularly imprinted polymer film

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Embodiment Construction

[0015] a. Weigh 0.25mmol of chloramphenicol in a flask, add 5ml of tetrahydrofuran to it, and sonicate for 5 minutes; Diol dimethacrylate was added into the flask, ultrasonicated for 5min;

[0016] b. Add 5 mg of initiator azobisisobutyronitrile, and sonicate for 15 minutes;

[0017] c. Add the solution obtained in step b to the 96-well ELISA plate dropwise, then put the 96-well ELISA plate into a sealed bag, fill it with nitrogen gas, seal it and irradiate it with a 100W UV lamp (λ=365nm) to initiate polymerization 6 hours;

[0018] d. Then place the 96-well ELISA plate in a methanol-acetic acid mixture with a volume ratio of 3:1, ultrasonically elute for 48 hours, and change the eluent every four hours to remove template molecules;

[0019] e. Dry the 96-well ELISA plate at 50°C for 2 hours to obtain a chloramphenicol molecularly imprinted polymer membrane.

[0020] test:

[0021] 1. Synthetic enzyme-labeled antigen (enzyme-labeled chloramphenicol):

[0022] Use glutar...

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Abstract

The invention discloses a preparation method of a 96-well enzyme label plate chloramphenicol molecularly imprinted polymer film. The preparation method comprises the following steps of a, dissolving chloramphenicol as a template molecule, N,N-diethylaminoethyl methacrylate as a functional monomer and ethylene glycol dimethacrylate as a cross-linking agent in tetrahydrofuran as a pore forming agent according to a mole ratio of 1: 4: 0.2, and carrying out ultrasonic treatment for 30min, wherein a use amount ratio of tetrahydrofuran to chloramphenicol is 5ml / 0.25mmol, b, adding azodiisobutyronitrile as an initiator into the mixture, and carrying out ultrasonic treatment for 15min, wherein a use amount ratio of azodiisobutyronitrile to chloramphenicol is 5mg / 0.25mmol, c, dropwisely adding the solution obtained by the step b on a 96-well enzyme label plate, putting the 96-well enzyme label plate into a sealing bag, filling nitrogen into the sealing bag, carrying out sealing, and carrying out ultraviolet lamp irradiation to initiate polymerization lasting for 6h, d, putting the 96-well enzyme label plate into a methanol-acetic acid mixed solution having a volume ratio of 3: 1, and carrying out ultrasonic elution for 48h, and e, drying the 96-well enzyme label plate at a temperature of 50 DEG C for 2h to obtain the chloramphenicol molecularly imprinted polymer film.

Description

technical field [0001] The invention relates to a method for preparing a molecularly imprinted polymer film, in particular to a method for preparing a chloramphenicol molecularly imprinted polymer film on a 96-hole microplate plate with high selective adsorption of chloramphenicol. Background technique [0002] Chloramphenicol (Chloramphenicol, CAP) is an antibiotic isolated from microbial metabolites for the first time in 1947. Due to its excellent antibacterial properties, stable drug properties, and low price, it has been widely used as a feed additive to treat bacterial diseases. However, due to the nitro group on its benzene ring, its decomposition half-life is long and has serious toxic side effects, which can easily cause human blood poisoning, lead to serious toxic side effects such as irreversible aplastic anemia, and constitute a huge potential for human health. threaten. Therefore, the European Union and the United States have stipulated in the regulations that t...

Claims

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Application Information

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IPC IPC(8): G01N33/531
Inventor 张峰杜幸洁张慧晓文永佳
Owner DALIAN OCEAN UNIV
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