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Detection method for residual amount of mancozeb in foodstuff

A technology of mancozeb and a detection method is applied in the field of mancozeb residue detection in food, which can solve the problems of cumbersome derivatization process, large consumption of reagents, incomplete reaction, etc., so as to reduce the use of drugs, The effect of saving cost and improving work efficiency

Inactive Publication Date: 2013-09-04
LAIYANG HENGRUN FOODSTUFF
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  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Mancozeb is a pesticide widely used in the world, and all countries have set strict residue standards in food; at present, the methods for the determination of mancozeb residues in food are mainly gas chromatography and liquid chromatography. These methods have a common disadvantage that mancozeb needs to be derivatized for detection, and the derivatization process is cumbersome, the reaction is incomplete, the derivation time is long, and the amount of reagents consumed is large, which cannot guarantee accurate detection results in a short time

Method used

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  • Detection method for residual amount of mancozeb in foodstuff
  • Detection method for residual amount of mancozeb in foodstuff
  • Detection method for residual amount of mancozeb in foodstuff

Examples

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Embodiment 1

[0024] Example 1, a method for detecting mancozeb residues in food is first to prepare EDTA solution, which is to weigh 74.4g EDTA-Na2, dissolve it with 600-700mL water, and adjust the pH with 1mol / L sodium hydroxide The value is 9.6-10, and the volume is adjusted to 1L with water; extraction: Weigh 20g of the crushed sample into a 250mL Erlenmeyer flask, add 100mL of prepared EDTA solution, adjust the pH value between 7.5-8.0 with 2mol / L hydrochloric acid, Ultrasonic extraction for 20 minutes, transferred to a centrifuge tube, centrifuged at 4000 r / min for 5 minutes, transferred the supernatant to a 250mL Erlenmeyer flask, and extracted the sample residue with 50mL LEDTA solution, the steps were the same as above, combined the two extracts and centrifuged, Take the supernatant and pass it through a 0.22 micron filter membrane to be tested; detection: instrument used: liquid chromatography inductively coupled plasma mass spectrometry (LC-ICP-MS), instrument model: liquid chroma...

Embodiment 2

[0027] Embodiment 2, a method for detecting mancozeb residues in food. In this example, green beans are used as the sample to be tested, and 20 g of mashed samples are weighed in a 250 mL conical flask, and 100 mL of the prepared EDTA solution is added. Adjust the pH value to 7.5-8.0, ultrasonically extract for 20min, transfer to a centrifuge tube, centrifuge at 4000 r / min for 5min, transfer the supernatant to a 250mL Erlenmeyer flask, then extract the sample residue with 50mL of EDTA solution, the steps are the same as above, Combine the two extracts and centrifuge, take the supernatant and pass it through a 0.22 micron filter membrane for testing, HPLC-ICP-MS working parameters: RF power: 1550 W, sampling depth: 10.0 mm, carrier gas, flow rate: argon 99.99%, 1.08L / min, nebulization temperature: 2°C, sample cone: platinum cone, analytical column: Agilent TC-C18 250 mm×4.6mm 5μm, flow rate: 0.6mL / min, injection volume: 20 μL; tuning mode: no gas mode, sample collection mode: ...

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Abstract

The invention discloses a detection method for a residual amount of mancozeb in foodstuff. The detection method comprise the steps: firstly, preparing EDTA solution, extracting a sample by the EDTA solution for two times, centrifuging after merging the two extracts, passing the centrifuging supernatant through a 0.22 microns filter membrane, and detecting by using a liquid chromatography inductively coupled plasma mass spectrometer (LC-ICP-MS). The detection method has the characteristics of speediness, accuracy, short detection period, and lower detection limit and higher accuracy degree.

Description

technical field [0001] The invention belongs to the technical field of food detection, and in particular relates to a detection method for mancozeb residues in food. Background technique [0002] Mancozeb is a pesticide widely used in the world, and all countries have set strict residue standards in food; at present, the methods for the determination of mancozeb residues in food are mainly gas chromatography and liquid chromatography. These methods have a common disadvantage that mancozeb needs to be derivatized for detection, and the derivatization process is cumbersome, the reaction is incomplete, the derivation time is long, and the amount of reagents consumed is large, which cannot guarantee accurate detection results in a short time . Contents of the invention [0003] The purpose of the present invention is to improve the deficiencies of the prior art and provide a method for detecting mancozeb residues in foods that is fast and accurate, shortens the detection peri...

Claims

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Application Information

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IPC IPC(8): G01N27/62G01N27/626
Inventor 任海涛管立辉周文峰
Owner LAIYANG HENGRUN FOODSTUFF
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