Method for preparing rose apple nucleolus polysaccharide

Abstract

The invention relates to a method for preparing rose apple nucleolus polysaccharide, which comprises the following steps: (1) after drying and crushing rose apple nucleoli, adding the dried and crushed rose apple nucleoli into petroleum ether solution, performing water-bath heating reflux, then adding ethanol to carry out reflux after extraction filtration, and drying after extraction filtration; (2) adding dried powder into water and processing solution by using ultrasonic waves; (3) after finishing ultrasonic processing, regulating the pH value, adding cellulase and then performing enzymolysis and enzyme killing; and (4) removing precipitates in the solution which is subjected to enzyme killing and is cooled, taking out supernatant to carry out concentration, then adding chloroform and n-butyl alcohol mixed solution, centrifuging, taking out supernatant to be added with absolute ethyl alcohol precipitate polysaccharide, performing extraction filtration after standing, taking out solid matter to be washed sequentially by acetone, diethyl ether and absolute ethyl alcohol, and after drying, obtaining rose apple nucleolus polysaccharide powder. According to the invention, an ultrasonic-assisted enzymic method is adopted to extract the rose apple nucleolus polysaccharide; and not only is the method good in extracting effect, but also extraction time can be effectively shortened and production efficiency is improved.

Description

technical field [0001] The invention relates to a preparation method of water apple nut kernel polysaccharide. Background technique [0002] Water apple, also known as Xiangguo, Fenggu, and water pomegranate, belongs to the family Myrtaceae, and is a tropical perennial evergreen tree. Native to India, Malay Peninsula and Andaman Islands. It was introduced into Taiwan, my country in the 17th century, and was introduced successively in Hainan, Guangdong, Guangxi, Fujian and Yunnan after the 1930s. [0003] The leaves, flowers, fruits and seeds of the apple apple can be used as medicinal materials. The leaves and flowers can be smashed to cure hemorrhoids. [0004] Since apple apple belongs to tropical and subtropical fruit trees, compared with economic crops that also grow in tropical and subtropical regions and other plants that grow in a wide area, there are relatively few studies on it. . [0005] In the current published literature, "Response Surface Surface Optimizati...

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Problems solved by technology

[0005] In the current published literature, "Response Surface Surface Optimization Research on the Extraction Process of Calambola Nucleolus Polysaccharides" records the test method for the crude polysaccharides of Calamaria polysaccharides. The extraction time is as long as nearly 3 hours, the extraction time is long, the production efficiency is low, and it is not conducive to industrial production