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Application of doxylamine succinate in preparing drug for treating or preventing influenza virus

A kind of technology of doxylamine succinate and prevention of influenza, which is applied in the field of medicine to achieve the effect of broad-spectrum antiviral activity

Inactive Publication Date: 2014-12-17
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently there is no direct application of doxylamine succinate in anti-influenza virus

Method used

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  • Application of doxylamine succinate in preparing drug for treating or preventing influenza virus
  • Application of doxylamine succinate in preparing drug for treating or preventing influenza virus
  • Application of doxylamine succinate in preparing drug for treating or preventing influenza virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Evaluation of anti-influenza virus activity of doxylamine succinate

[0029] 1. Experimental materials and methods

[0030] 1.1 Cells, viruses and drugs

[0031] MDCK cells were purchased from ATCC, virus A / PR8 / 34H1N1 was amplified from chicken embryo culture, and doxylamine succinate was purchased from Sigma;

[0032] 1.2 Experimental Instruments

[0033] Multifunctional detector PerkinElmer, inverted microscope Costar

[0034] 2. experimental method:

[0035] 2.1 Cell culture:

[0036] 37°C, 5% (volume ratio, the same below) CO 2 cultured in a humidified incubator. A DMEM medium containing 10% (volume ratio, the same below) FBS, 100 U / mL of penicillin and streptomycin was used. Cells were subcultured after reaching 90% confluence, and the subculture ratio was 1 / 3–1 / 4.

[0037] 2.2 Virus culture:

[0038] Take 9-11 day-old SPF chicken embryos, check them with an egg tester before inoculating the virus, and mark them at a position far away from ...

Embodiment 2

[0053] Example 2: Broad-spectrum research on anti-influenza virus activity of doxylamine succinate

[0054] In this experiment, the antiviral activity of doxylamine succinate on influenza A virus subtype H1N1 strain, influenza A virus H3N2 strain and influenza B virus strain was detected.

[0055] 1. The virus strains used in the experiment include: A / human / Hubei / 1 / 2009 (H1N1), A / human / Hubei / 3 / 2005 (H3N2), Influenza B virus

[0056] 2. Divide MDCK cells into 2*10 4 Cells / well were seeded in 96-well cell culture plates, cultured in a 37°C cell culture incubator for 14-18 hours, and the cells were grown into a single layer before use. Discard the culture medium in the well plate, wash it twice with PBS, add 100TCID50 / well virus solution to infect the cells, and add drugs with different concentration gradients at the same time (with 500μM as the initial concentration, serially dilute 8 gradients by 2 times, each gradient 2 A total of 200 μl of maintenance medium (DMEM+0.3%BSA...

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Abstract

The invention discloses application of doxylamine succinate in preparing a drug for treating or preventing influenza virus. The application comprises the following steps of: selecting the doxylamine succinate with complete non-toxic concentration to carry out antiviral experiment, wherein the result shows that the micromolecule compound has remarkable antiviral activity and is dose-dependent; and detecting the antiviral activity of the doxylamine succinate to different types and subtypes of influenza viruses, wherein the result indicates that the doxylamine succinate has activity for detecting virus strain, a dose-dependent effect, as well as a certain broad-spectrum to the anti-influenza virus activity. Therefore, the doxylamine succinate disclosed by the invention can be developed as a novel anti-influenza virus drug, so that a novel way and means is provided for treating the influenza.

Description

technical field [0001] The invention belongs to the technical field of medicine, and more specifically relates to an application of doxylamine succinate in the preparation of medicines for treating or preventing influenza virus. Background technique [0002] Influenza virus is a pathogen that seriously affects human health and can cause influenza. According to the differences in the antigenic and genetic characteristics of the virion nucleoprotein (NP) and matrix protein (M), influenza viruses are divided into three types: A, B, and C, also known as A, B, and C. There are significant differences in the genome structure, polypeptide composition, infectivity and pathogenicity of these three different types of viruses. The whole genome of influenza A virus consists of eight single-stranded negative-strand RNAs of different sizes, named after segment 1 to segment 8, respectively. The total length of the viral genome is about 13.6kb, encoding 10 structural proteins (PB2, PB1, P...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/4402A61P31/16
Inventor 陈绪林廖庆姣安利伟
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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