Producing method of streptomycin antibiotic molecular imprinting biosensor

Abstract

The invention relates to a producing method of a streptomycin antibiotic molecular imprinting biosensor. The producing method comprises the following steps of: 1, pretreating a glassy carbon electrode; 2, modifying an electrode by a molecular self-assembled film, namely, immersing the treated electrode in a polymerization base solution, and after introducing pure N2 for 10min, scanning for 20 circles by cyclic voltammetry within a potential interval of 0-0.8V, wherein the scanning speed is 0.05V / s; and 3, eluting a template molecule, namely, modifying the electrode by adopting the step 2, and then eluting through 70wt% of alcohol solution to obtain the streptomycin antibiotic molecular imprinting biosensor. Based on a molecular imprinting technology, by using an electrochemical polymerization method and a cyclic voltammetry method, a molecular imprinting electrode is produced from streptomycin sulphate as a template molecule and o-phenylenediamine as a functional monomer, a rapid and sensitive streptomycin detection method is established, and the basis is provided for small, automatic and low-detection-limit electrochemical sensors.

Description

technical field [0001] The invention belongs to the field of chemical analysis, and relates to a biosensor, in particular to a preparation method of a streptomycin antibiotic molecularly imprinted biosensor. Background technique [0002] Streptomycin (STR) is an aminoglycoside antibiotic that has significant antibacterial activity against Gram-negative bacteria and some Gram-positive bacteria, especially Mycobacterium tuberculosis. It is used as feed in animal husbandry and aquaculture Additives and treatments for animal diseases are widely used. However, streptomycin has obvious toxic effects on the auditory nerve, can cause deafness, is more serious for allergic fetuses, and has nephrotoxicity. Streptomycin residues caused by abuse, non-compliance with the withdrawal period, and overdose of medication will directly threaten Human and animal health. STR residues can be found in meat, liver, kidney, milk and honey. All countries have made clear regulations on STR residue ...

AI Technical Summary

Problems solved by technology

Although some physical and chemical detection methods are more sensitive and accurate, because streptomycin lacks a strong UV-absorbing chromogenic group, it often needs to be processed by a series of derivatization methods before detection, which is cumbersome and time-consuming; high performance liquid chromatography The use of large-scale equipment such as instruments and mass spectrometers has high requirements for inventors and high detection costs

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