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Carrier system and method for inputting nanoparticles into cell

A nanoparticle and carrier system technology, applied in the field of nanomaterials, can solve problems such as toxicity, avoid cell membrane rupture, and improve aggregation efficiency.

Inactive Publication Date: 2013-08-14
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this approach has specific features and limitations, and a series of hurdles must be overcome to achieve efficient delivery of nanoparticles in target cell culture, avoiding toxic effects in target cells and being able to efficiently deliver nanoparticles and nanoparticle in cells. Particle-loaded functional ingredients

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0044] A carrier system for introducing nanoparticles into cells, characterized in that the carrier system is lipid microbubbles, which are prepared by the following steps:

[0045] (1) Evenly mix egg yolk lecithin EPC in chloroform solvent to prepare a mixed solution;

[0046] (2) Place the mixed solution in a rotary evaporating drier at 30°C to evaporate to dryness, and then place it in a desiccator to remove trace solvents to obtain a dry lipid film;

[0047] (3) Add the dried lipid film to phosphate buffer, place in a shaker and vibrate for 30 min-2 h to hydrate the lipid film;

[0048] (4) Place the hydrated lipid film in an ultrasonic cavitation instrument, and conduct ultrasonic cavitation for 10-60 min while passing nitrogen gas into the hydrated lipid film. The ultrasonic cavitation power is 80-180 W. Obtain microbubble solution;

[0049] (5) Wash the microbubble solution with phosphate buffer, then add avidin, and shake at 0°C for 30 min-2 h; after washing with pho...

Embodiment 2

[0055] A carrier system for introducing nanoparticles into cells, characterized in that the carrier system is lipid microbubbles, which are prepared by the following steps:

[0056] (1) Uniformly mix lecithin PC and soybean lecithin in chloroform solvent to prepare a mixed solution;

[0057] (2) Place the mixed solution in a rotary evaporating drier at 30°C to evaporate to dryness, and then place it in a desiccator to remove trace solvents to obtain a dry lipid film;

[0058] (3) Add the dried lipid film to phosphate buffer, place in a shaker and vibrate for 30 min-2 h to hydrate the lipid film;

[0059] (4) Place the hydrated lipid film in an ultrasonic cavitation instrument, and conduct ultrasonic cavitation for 10-60 min while passing argon gas into the hydrated lipid film, and the ultrasonic cavitation power is 80-180 W , to obtain microbubble solution;

[0060] (5) Wash the microbubble solution with phosphate buffer, then add avidin, shake at 0°C for 30 min-2 h; after w...

Embodiment 3

[0066] A carrier system for introducing nanoparticles into cells, characterized in that the carrier system is lipid microbubbles, which are prepared by the following steps:

[0067] (1) Uniformly mix lecithin (PC), pegylated distearoylphosphatidylcholine (DSPE-PEG), and cholesterol in chloroform solvent to prepare a mixture;

[0068] (2) Place the mixed solution in a rotary evaporating drier at 30°C to evaporate to dryness, and then place it in a desiccator to remove trace solvents to obtain a dry lipid film;

[0069] (3) Add the dried lipid film to phosphate buffer, place in a shaker and vibrate for 30 min-2 h to hydrate the lipid film;

[0070] (4) Place the hydrated lipid film in an ultrasonic cavitation instrument, and conduct ultrasonic cavitation for 10-60 min while passing oxygen into the hydrated lipid film, and the ultrasonic cavitation power is 80-180 W. Obtain microbubble solution;

[0071] (5) Wash the microbubble solution with phosphate buffer, then add avidin, ...

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Abstract

The invention relates to a carrier system for inputting nanoparticles into a cell. The carrier system is prepared through the following steps: preparing a lipid membrane from one or multiple phospholipids, performing ultrasonic cavitation on the hydrated lipid membrane to obtain a microbubble solution, and finally adding avidin and a cell specific antibody. The invention also relates to a method for inputting nanoparticles into a cell, which comprises the following specific steps: adsorbing nanoparticles on the surface of the microbubble of the carrier system, adding a cultured cell, and finally performing ultrasonic treatment on a microbubble-adsorbed cell solution in an ultrasonic field. According to the invention, nanoparticles are efficiently input into a cell without any loss through the cavitation effect of the microbubble, thus providing a carrier system and method for safely, conveniently, accurately and quantitatively inputting various nanoparticles into a cell for research of nano biomedicine.

Description

technical field [0001] The invention relates to the field of nanomaterials, in particular to a method for in vitro or in vitro induction by ultrasonic waves, using the cavitation effect of microbubbles to transport nanoparticles into cells in a non-destructive and efficient manner, providing safe and convenient nano-biomedical research. 1. A carrier system and method for accurately and quantitatively controlling the delivery of various nanoparticles into cells. Background technique [0002] The impact of functional nanomaterials on biomedicine has far-reaching significance, and the development of nanobiomedicine depends to a large extent on the development of nanomaterials. There are many types of nanomaterials in nanobiomedical applications, and their specific functions and application purposes are also diverse. For example, they have broad application prospects in medical image contrast enhancement, tissue repair, immunoassay, drug delivery, and cell sorting. The wide ran...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12N5/078
Inventor 杨芳顾宁
Owner SOUTHEAST UNIV
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