Marek's disease virus and subgroup-J avian leukosis virus rapid combined-detection test strip

Abstract

The invention discloses a test strip used in one-step rapid detection of Marek's disease virus (MDV) and subgroup-J avian leukosis virus (ALV-J). The test strip comprises a non-absorbing supporting layer, and an absorption layer adhered to the supporting layer. The absorption layer is formed by sequentially spliced components of an absorption fiber layer, a gold-labeled antibody fiber layer, a cellulose film layer, and a water absorption layer. The cellulose film layer is marked with anti-goat IgG or anti-mouse IgG control blot, and a detection blot comprising anti-MDV and anti-ALV-J antibody. The anti-MDV and anti-ALV-J antibody is an anti-MDV and anti-ALV-J antibody monoclonal or polyclonal antibody. An anti-MDV and anti-ALV-J mixed polyclonal antibody or monoclonal antibody marked by colloidal gold and corresponding to the detection blot is adhered to the gold-labeled antibody fiber layer. The test strip provided by the invention has the advantages of high detection specificity, high sensitivity, simple operation, fast detection, and intuitive result. The test strip is suitable for MDV and ALV-J virus on-site rapid combined detection, and can be used in identification and diagnosis of single infection or mixed infection of the two viruses. The test strip can be widely applied, and is suitable for popularization.

Description

[0001] technical field [0002] The invention relates to a device for detecting Marek's disease and J subgroup avian leukemia virus, in particular to a colloidal gold test strip for rapid detection of Marek's disease virus and J subgroup avian leukemia virus in one step. technical background [0003] Marek's disease (Marek's disease, MD) is a highly contagious, contagious, lymphoproliferative neoplastic disease of poultry caused by Marek's disease virus (MDV), mainly harming chickens, quail and pheasant also sickness. The disease can cause neuroswelling of lymphocytomas of internal organs, muscles, and peripheral nerves. Clinically, MD-affected chicken flocks show symptoms such as uneven growth, extreme emaciation, and death. The most common lesions are nerve damage and lymphatic tumors of various solid organs, with liver and spleen tumors being the most common. Asymmetric progressive paralysis, eventually leading to complete paralysis of one or more extremities. MDV belo...

AI Technical Summary

Problems solved by technology

The detection cost of this kind of method is relatively high, and the test operation is more complicated, and it is difficult for general farm staff to master

Molecular biology methods such as PCR, RT-PCR, and fluorescent quantitative PCR are mainly used for the detection of viral nucleic acids. Although these techniques can accurately differentiate MDV and ALV-J, the operation is complicated, requires specific reagents and is expensive. Due to the variation of ALV-J antigen, ordinary PCR detection, especially those molecular biology methods based on env gene sequence, need to be revised regularly Specific primers to ensure detection of new mutant strains

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