Marek's disease virus and avian reticuloendotheliosis virus rapid joint detection test strip

Abstract

The invention discloses a test strip for quickly detecting Marek's disease virus MDV and poultry reticuloendothelial disease virus REV by one-step method, which includes a non-absorbent support layer and an adsorption layer attached to the support layer. The adsorption layer is composed of sample adsorption fibers Layer, gold-labeled antibody fiber layer, cellulose film layer and water-absorbing layer are sequentially spliced; the cellulose film layer is labeled with anti-goat IgG or anti-mouse IgG control blots, and antibody detection blots containing anti-MDV and anti-REV The anti-MDV and anti-REV antibodies are anti-MDV and anti-REV monoclonal antibodies or polyclonal antibodies; the gold-labeled antibody fiber layer is attached with colloidal gold-labeled anti-MDV and anti-REV corresponding to the detection blot mixed polyclonal or monoclonal antibodies. The test strip detection has strong specificity, high sensitivity, easy operation, rapid detection, and intuitive results, and is suitable for on-site rapid joint detection of MDV and REV viruses, and can also be used for differential diagnosis of separate infection or mixed infection of the two viruses . The test strip has a wide application range and is convenient for popularization and application.

Description

technical field [0001] The invention relates to a device for detecting Marek's disease and poultry reticuloendotheliosis, in particular to a colloidal gold test strip for rapid detection of Marek's disease virus and poultry reticuloendotheliosis virus in one step. technical background [0002] Marek's disease (Marek's disease, MD) is a highly contagious, contagious, lymphoproliferative neoplastic disease of poultry caused by Marek's disease virus (MDV), mainly harming chickens, quail and pheasant also sickness. The disease can cause neuroswelling of lymphocytomas of internal organs, muscles, and peripheral nerves. Clinically, MD-affected chicken flocks show symptoms such as uneven growth, extreme emaciation, and death. The most common lesions are nerve damage and lymphatic tumors of various solid organs, with liver and spleen tumors being the most common. Asymmetric progressive paralysis, eventually leading to complete paralysis of one or more limbs. MDV belongs to the he...

AI Technical Summary

Problems solved by technology

The detection cost of this method is relatively high, and because the REV infection in some cases is "transient", the viremia time is short, and the virus content is low, the detection rate is low

Molecular biology methods such as PCR, fluorescent quantitative PCR and LAMP are mainly used for the detection of viral nucleic acids. Although these technologies can accurately differentiate MDV and REV, the operation is complicated and requires specific reagents and expensive equipment. The operation requirements are high, and it is difficult to meet the needs of rapid differential diagnosis on the production line or on the spot of epidemic diseases

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