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Avian reticuloendothelial disease virus rapid detection test strip

A technology of poultry mesh endothelium and test strips, which is applied to measurement devices, instruments, scientific instruments, etc., can solve problems such as being unsuitable for the production line of rapid diagnosis, complex detection technology operation, and time-consuming detection process, and is not easy to false negative. and false positives and misjudgments, strong detection specificity, and intuitive results display

Active Publication Date: 2016-05-11
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] REV's virus isolation and culture, PCR, fluorescent quantitative PCR and LAMP detection techniques are complicated to operate, require expensive equipment, and the detection process takes a long time, which is not suitable for the rapid diagnosis needs of the production line or the epidemic site
However, AGP, IFA, ELISA and other methods can only be used for the detection of REV antibodies
Although the ELISA assay method has the characteristics of specificity and sensitivity, the operation is complex, time-consuming and labor-intensive, requiring specific equipment and professional technicians to operate, and it is difficult to popularize at the grassroots level

Method used

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  • Avian reticuloendothelial disease virus rapid detection test strip
  • Avian reticuloendothelial disease virus rapid detection test strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: A test strip for rapid detection of poultry reticuloendotheliosis virus, see figure 1 with figure 2 , the support layer 1 is made of plastic sheet strips, the sample adsorption fiber layer 2 is made of glass wool, and the gold-labeled antibody fiber layer 3 is attached with colloidal gold-labeled anti-REV monoclonal antibodies made of glass wool, The cellulose membrane layer 4 is made of nitrocellulose, and the water-absorbing layer 5 is made of water-absorbing filter paper. The layers numbered 2, 3, 4, and 5 are pasted on the support layer 1 in sequence, and the fibers at the joints where they are spliced ​​intersect and penetrate each other. The cellulose membrane layer 4 is provided with an anti-REV polyclonal antibody IgG solution to mark a detection blot 6 (code T), and a goat (rabbit) anti-mouse IgG solution to mark a control blot 7 (code C); the detection blot and the control blot are arranged in the form of " ｜｜ ". The test end protective film 8-...

Embodiment 2

[0052] Example 2: The test strip for detecting reticuloendothelial disease virus is basically the same in structure and preparation method as in Example 1, except that the gold-labeled antibody fiber layer 3 made of nylon fibers is attached with colloidal gold-labeled anti- REV gold-labeled polyclonal antibody; on the cellulose membrane layer 4 made of nitrocellulose, there is a detection blot T sprayed with anti-REV monoclonal antibody IgG solution, and a control blot C sprayed with goat (rabbit) anti-mouse IgG solution . Others including detection sample preparation, operation method and result judgment etc. are all the same as Example 1.

Embodiment 3

[0053] Example 3: Detect reticuloendothelial disease virus test strip, its structure, preparation method are basically the same as embodiment 1, the difference is: on the gold label antibody fiber layer 3 that is made of polyester fiber, be attached with anti-REV gold label sheet Cloned antibody; on the cellulose membrane layer 4 made of polyvinylidene fluoride, there is a detection blot T labeled with the corresponding anti-REV polyclonal antibody IgG solution, and a control blot labeled with goat (rabbit) anti-mouse IgG solution c. Others including detection sample preparation, operation method and result judgment etc. are all the same as Example 1.

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Abstract

The invention discloses a test strip for rapidly detecting poultry reticuloendothelial hyperplasia virus REV, which comprises a non-absorbent support layer and an adsorption layer attached to the support layer. The adsorption layer consists of a sample adsorption fiber layer and a gold-labeled antibody fiber layer. The cellulose membrane layer and the water-absorbing layer are spliced ​​in sequence, and the control blot of goat anti-mouse IgG is marked on the cellulose membrane layer, and the detection blot containing anti-REV antibody; the anti-REV antibody is an anti-REV monoclonal antibody or polyclonal antibody Cloned antibody: The colloidal gold-labeled anti-REV polyclonal antibody or monoclonal antibody corresponding to the detection blot is attached to the gold-labeled antibody fiber layer. The test strip has strong specificity and high sensitivity, simple and fast operation, intuitive and accurate result display, no need for additional equipment and reagents, low detection cost, and is suitable for rapid detection of poultry reticuloendothelial disease virus; use Wide range, easy to popularize and apply.

Description

technical field [0001] The invention relates to a device for detecting poultry reticuloendothelial disease virus, in particular to a colloidal gold test strip for rapidly detecting poultry reticuloendothelial disease virus. technical background [0002] Avian reticuloendotheliosis (Reticuloendotheliosis, RE) is a group of chickens, ducks, geese, turkeys and other poultry caused by avian reticuloendotheliosis virus (Reticuloendotheliosis virus, REV) with the main feature of reticuloendotheliosis syndromes, including acute reticulosis, short stature syndrome, and chronic neoplastic proliferative disorders of lymphoid and other tissues. RE is one of the most important immunosuppressive and neoplastic diseases of poultry. The natural hosts of REV infection include turkeys, chickens, ducks, geese and Japanese quail, among which turkeys are most susceptible to infection, and some virus strains are more pathogenic to ducks than chickens. REV mainly infects chicks, especially newl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569
Inventor 张改平罗俊滕蔓崔治中邓瑞广江国托赵东赵鹏柴书军程娜胡骁飞职爱民杨苏珍卢清侠郝慧芳
Owner HENAN ACAD OF AGRI SCI
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