Preparation method of protein short peptide chelated calcium

Abstract

The invention provides a preparation method of protein short peptide chelated calcium. The method includes the steps of: refinement treatment and sterilization of mixed raw material containing protein and water, a first hydrolysis by adding proteolytic enzyme, ultrafiltration of the liquid portion separated after the first hydrolysis using a ultrafilter membrane whose retention molecular weight is in the range of 1000-6000 Da, mixing of the macro-molecule peptide liquid which does not permeate the ultrafilter membrane and the solid phase portion separated by the first hydrolysis, a second hydrolysis by adding proteolytic enzyme, purification of micromolecule oligopeptide liquid after twice hydrolysis ultrafiltrations, chelating by adding calcium hydrate or calcium chloride, complete reaction of quantitive sodium carbonate when the pH valve is neutral, filtering for removing deposition, desalination by the nanofiltration membrane, sterilization, condensation and obtaining of the protein short peptide chelated calcium product. The preparation method can substantially improve the protein short peptide obtaining rate and the raw material protein utilization rate, and reduce production cost, so the obtained protein short peptide chelated calcium product has a good quality and high production safety.

Description

technical field [0001] The invention relates to a chelated calcium, in particular to a preparation method of a protein short peptide chelated calcium. Background technique [0002] Calcium is one of the most abundant mineral elements in the human body, and it is also the most active element in the body. It has a variety of important physiological functions. It is an essential component for maintaining the normal excitation of nerves and muscles, and it is also necessary for ensuring bone growth and maintaining bone hardness. substance. Then, calcium deficiency belongs to global health. According to the results of the latest "Nutrition and Health Status Survey of Chinese Residents", about 97% of the population in my country is deficient in calcium. The national average per person per day is only 391mg. Calcium deficiency is relatively common among children, women and the elderly. According to data, the average prevalence of rickets in children under 3 years old in some areas...

AI Technical Summary

Problems solved by technology

[0007] In the existing disclosed protein peptide chelated calcium preparation methods, all include the preparation method of protein peptide, but there are the following deficiencies: one is that the influence of the molecular weight of the peptide on the properties of chelated calcium is not considered, such as water solubility, in the protein peptide In the preparation of chelated calcium, after the peptide with large molecular weight forms chelated calcium, its water solubility decreases and precipitation occurs, which will affect the absorption of chelated calcium in the body. At present, in the existing protein peptide preparation methods, The proportion of protein peptides, especially short peptides such as 2-peptides and 3-peptides is low, and the proportion of large molecular weight peptides is relatively high, which affects the water solubility of the product and the bioavailability of chelated calcium; second, the existing protein peptides In the preparation method, there is no or little consideration of the yield of protein peptides, and the preparation rate of protein short peptides is low, which will increase the cost of raw materials for production, and the same protein raw materials will also increase the productivity of protein short peptide chelated calcium. Reduced, high product cost, increasing the burden on consumers

In this way, in industrial production, a large amount of alcohol needs to be used, the production cost is high, and there is a risk of flammability and explosion of organic solvents