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Arthrobacter strain highly effectively degrading phenanthrene, and application thereof

A technology of Arthrobacter and strains, which is applied to Arthrobacter strains that efficiently degrade phenanthrene and its application fields, can solve the problems of refractory degradation of polycyclic aromatic hydrocarbons, achieve the effect of eliminating harm to the human body and restoring ecological functions

Active Publication Date: 2013-07-24
PETROCHINA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, soil is a very complex environmental system, which is affected by many factors, such as nutrient components (carbon, nitrogen, phosphorus sources, etc.), temperature, oxygen partial pressure, water content, pH value or changes in salt concentration, etc., as well as microorganisms. There are non-culturable states in the environment and the refractory degradation of PAHs. At present, there is still no solid-phase microbial degradation technology suitable for its own system in China.

Method used

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  • Arthrobacter strain highly effectively degrading phenanthrene, and application thereof
  • Arthrobacter strain highly effectively degrading phenanthrene, and application thereof
  • Arthrobacter strain highly effectively degrading phenanthrene, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Isolation and Identification of Arthrobacter sp.

[0046] Inoculate the petroleum aromatics contaminated soil suspension in the loess plateau area with 15% inoculum amount in MS liquid medium with phenanthrene (200mg / L) as the sole carbon source, and culture it on a shaking table at 150r / min under aerobic conditions at 28°C for 3~ 4d, transfer once under the same conditions. Dilute and apply to MS solid medium with phenanthrene as the only carbon source, and culture at a constant temperature of 28°C for 4-5 days to form a single colony. Pick colonies that grow rapidly, plump, and with neat edges and put them into MS liquid medium, shake the flask for re-screening, and obtain the target strain.

[0047] Isolation of strain D4:

[0048] In this example, a total of 56 strains were isolated and purified, and it was found that one strain had the strongest degradation ability and the most vigorous growth. In the present invention, this strain was named D4 for further identi...

Embodiment 2

[0054] Example 2 Oily sludge microbial in-situ remediation treatment

[0055] In this example, the in-situ remediation treatment of oily sludge was carried out using the CGMCC No.6581 and / or indigenous bacteria screened in Example 1. The specific operations are as follows:

[0056] (1) Enrichment, acclimatization, activation and cultivation of the native bacteria of the oily sludge soil in the repaired well site and the CGMCC No.6581 of the present invention on the preservation medium, large-scale production, and standby;

[0057] Among them, the strain preservation medium: beef extract peptone slant medium, the specific formula is as follows:

[0058] Beef extract 5g, egg white 10g, sodium chloride 5g, agar 20g, distilled water 1000mL, pH7.2~7.4.

[0059] Expanded cultivation of bacterial agents: cultivate the Arthrobacter phenanthritis CGMCC No.6581 obtained in Example 1 and the indigenous bacteria Pseudomonas aeruginosa, Bacillus megaterium, Acinetobacter rufia, Nocardioid...

Embodiment 3

[0070] Example 3 Oily sludge microbial in-situ remediation treatment

[0071] In this example, the enrichment, acclimatization and activation of Arthrobacter phenatus CGMCC No.6581 used can be seen in Example 1. After activation and primary expansion, the bacteria were inoculated into MS liquid medium and expanded to 1×10 8 ~10 10 cfu.ml -1 For field repairs. The formula of the nutrient solution used in this embodiment is the same as the formula of the nutrient solution used in Example 1. In this embodiment, the specific operation of in-situ remediation treatment of oily sludge by using the phenanthrene bacteria is as follows:

[0072] Establish a 20*10*0.5m oily sludge stacking point in the trench operation area of ​​Longdong Oilfield, Changqing Oilfield 3 (Length*Width*Height) oily sludge biological treatment unit, 60m of oil-contaminated soil collected from drilling, oil testing, fracturing, well washing, maintenance and other operations 3 As for the processing unit, d...

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Abstract

The invention provides an arthrobacter strain highly effectively degrading phenanthrene, and an application thereof. The phenanthrene-degrading arthrobacter strain has a preservation number of CGMCC No. 6581. The strain provided by the invention can effectively degrade polycyclic aromatic hydrocarbon and heterocyclic substances in soil and oil sludge, and can be effectively applied in biodegradation treatments of petroleum-contaminated soil and PAHs pollutants in soil in loess highlands. Especially a phenanthrene degradation rate can be higher than 98%. The invention also provides a formula of a nutrient solution for the growth and degradation of the strain, and a method for applying the strain in petroleum-contaminated soil field reparation.

Description

technical field [0001] The invention belongs to the fields of energy biotechnology and environmental biotechnology. Specifically, the invention relates to a strain of Arthrobacter sp. and its application. The strain can effectively degrade polycyclic aromatic hydrocarbons and heterocyclic substances in soil and oil sludge, especially It is a phenanthrene substance, which can be effectively applied to the biodegradation treatment of petroleum-contaminated soil and PAHs pollutants in the loess plateau area. Background technique [0002] With the development of the petroleum industry, the pollution of petroleum to the environment is becoming more and more serious. In particular, the corrosion and leakage of underground oil tanks and oil pipelines pollute soil and groundwater sources, which not only cause soil salinization and poisoning, but also lead to soil damage and waste, and its toxic substances (mainly hydrocarbons, according to alkanes, naphthenes, alkenes, etc.) , The o...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02B09C1/10C02F11/02C12R1/06A62D101/20C02F101/32
Inventor 杨琴张海玲周立辉任建科冀忠伦赵敏任小荣李岩蒋继辉张璇
Owner PETROCHINA CO LTD
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