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Tannase high-producing strain and preparation method thereof

A high-yield strain and tannase technology, applied in the field of microorganisms, can solve the problem of low production efficiency of tannase

Inactive Publication Date: 2013-07-24
曹庸
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to address the above existing problems, to provide a tannase high-yield bacterial strain and its production method, thereby improving the tannase fermentation yield and solving the problem that the current tannase production efficiency is not high

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  • Tannase high-producing strain and preparation method thereof
  • Tannase high-producing strain and preparation method thereof

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Embodiment Construction

[0037] A high-yielding tannase strain, named T3-5-1, was identified as Aspergillus niger ( Aspergilluse niger ), the preservation number is CGMCC No.7423, the preservation time: April 7, 2013, and the preservation unit: General Microorganism Center (CGMCC) of China Committee for the Collection of Microorganisms.

[0038] T3-5-1 tannase high-yielding bacterial strain is obtained by screening and preparing in the following steps:

[0039] 1. Acquisition and preliminary screening of tannase-producing strains. Pick 10 g of rotten Chinese gallnuts and add 90 mL of sterile water, shake well, that is 10 -1 solution, serially diluted to 10 -10 , each gradient was coated with 3 plates on the screening medium, cultured at 30 °C for 48 h, and the single colonies with larger colonies and larger hydrolysis circles on the plates were picked and stored on the slant of the test tube PDA. Wherein the screening medium consists of: potassium dihydrogen phosphate 4.38g, ammonium sulfate 8.76g,...

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Abstract

The invention provides a tannase high-producing strain and a preparation method thereof. The preparation method comprises the following steps of: taking decayed gallnuts in nature as raw material, screening microorganisms in the decayed gallnuts in a culture medium with gallotannic acid as the only carbon source to obtain a strain T3 which is high in tannase production activity, performing 60 Co gamma ray irradiation induced mutation on the strain, and finally obtaining a strain having a number of T3-5-1 through flat-plate primary screening and shake flask fermentation re-screening, wherein the tannase production activity of the strain is increased by 122.12% in contrast with that of the strain T3. Through strain identification, the strain T3-5-1 is aspergilluseniger and the conservation number of the strain T3-5-1 is CGMCC No. 7423. The strain provided by the invention is capable of producing tanase under optimal cultivation condition; the specific activity of the strain reaches up to 5304.53 U / mg after the strain is separated and purified, which is far higher than the specific activity of 1788.15 U / mg of the tannase produced by American Sigma.

Description

[0001] technical field [0002] The invention relates to the technical field of microorganisms, in particular to the related fields of microorganism screening, mutation breeding, strain identification and fermentation enzyme production, and in particular provides a high-yielding strain of tannase and a preparation method thereof. Background technique [0003] Tannase (Tannase, E.C. 3.1.1.20), the full name of Tannin Acyl Hydrolase (Tannin Acyl Hydrolase), is an induced enzyme widely present in microorganisms and plants, especially in filamentous fungi, with tannin , Gallnut, etc. can be produced in large quantities when used as inducers. They belong to cell membrane-bound enzymes and can be secreted extracellularly. Tannase can specifically hydrolyze the ester bonds and depsyl carboxyl bonds in tannins to generate gallic acid, glucose and corresponding alcohols and other substances. [0004] Tannase has been used in many fields such as beverages, brewing, food, medicine, ch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N13/00C12N15/01C12R1/685
CPCC12N9/18C12Y301/0102C12N1/145C12R2001/685
Inventor 曹庸张帅林健辉农嘉仪朱华伟
Owner 曹庸
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