Usage of potassium ions in controlling metamorphosis of mytilus coruscus larvae
A technology of thick-shelled mussels and potassium ions, which is applied in the field of chemical ions potassium ions, to achieve the effects of shortening the time of attaching seedlings, increasing the metamorphosis rate, and cheap prices
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Embodiment 1
[0028] 1 Experimental materials
[0029] Sexually mature mussels were collected from Lycium barbarum, Shengsi County, Zhejiang; potassium chloride, potassium sulfate, and tetraethylamine were purchased from Sinopharm Chemical Reagent Co., Ltd.
[0030] 2 Experimental methods
[0031] 2.1 Cultivation of eye-spot larvae of thick-shelled mussel
[0032] Collect the sexually mature individuals of the thick-shelled mussels, brush off the sludge and barnacles attached to the body surface of the thick-shelled mussels with tools, and rinse them with sea water. After drying in the shade for 24 h, transfer to a 10 L polycarbonate container filled with filtered seawater (GF / C: 1.2 μm filter membrane, FSW: filtered seawater) at 20 °C. Adults begin to release sperm and eggs, which are stirred to fertilize. Fertilized eggs were placed in an incubator at 18°C for 2 days in the dark. After 2 days, the fertilized eggs develop into D-shaped larvae, and the larvae are cultivated in a beake...
Embodiment 2
[0050] This embodiment tests the influence of potassium ion solution on the metamorphosis rate and mortality of larvae when the potassium ion solution is exposed to 1h, 8h, and 16h respectively at a concentration of 50 mM, and the effect of tetraethylamine solution on 1 mM concentration. The effect on the metamorphosis rate and mortality of the larvae when the larvae were treated for 30 minutes, 45 minutes and 60 minutes, the specific operation was the same as in Example 1.
[0051] The potassium ion solution was exposed to 50 mM concentration of mussel larvae for 1 h, 8 h, and 16 h, respectively, and then transferred to filtered seawater for cultivation. The results of larval metamorphosis and mortality after 96 h are shown in Table 1 and Table 2.
[0052] Table 1 Metamorphosis rate of larvae after 96 h incubation under different treatment conditions of potassium ion solution
[0053]
[0054] Table 2 Mortality of larvae incubated for 96 h under different treatment conditi...
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