Tissue culturing and rapid propagating method for chloranthy florida var. plena

A technology for tissue culture and clematis, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of slow reproduction speed and restrictions on the production and development of precious species of green-flowered clematis

Inactive Publication Date: 2013-07-17
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Clematis double petals can not produce seeds, the propagation method is layering, division or cutting, the propagati

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A kind of Clematis double petals tissue culture fast propagation method, concrete steps are as follows:

[0019] 1. Explant treatment and inoculation

[0020] Collect the stem tips of Clematis double petals explants, wash them with sterile water on an ultra-clean bench, treat them with 75% alcohol for 30 seconds, soak them in 0.1% mercury liter for 10 minutes, rinse them with sterile water Shoot tips were inoculated in cluster bud induction medium. After the cluster buds are produced, transfer them to the cluster bud proliferation medium for further cultivation to continuously expand the number of seedlings. When large seedlings are needed, the 2-3 cm long cluster buds are cut off and then transferred to the strong seedling rooting medium for cultivation, that is, they grow into test-tube plantlets of Clematis double petals with roots.

[0021] 2. Culture medium formula: According to different growth stages, the best formula of the culture medium is as follows:

[0...

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Abstract

The invention provides a tissue culturing and rapid propagating method for chloranthy florida var. plena. The tissue culturing and rapid propagating method comprises the following steps of: (1) washing stem tips of caulicles of chloranthy florida var. plena with sterile water on an ultra-clean table, immersing the washed stem tips by 75% of alcohol for 20-30 seconds, immersing and disinfecting the stem tips by 0.1%-0.2% of mercury bichloride for 6-10 minutes, and inoculating the stem tips, which are washed by the sterile water, into a cluster bud induction medium for primary induction culturing; (2) dividing cluster buds obtained through primary induction culture into small individual buds, and inoculating the small individual buds into a cluster bud propagation culture medium for subculturing; and (3) inoculating bud seedlings into a strong seedling rooting culture medium for rooting culturing when the bud seedlings grow to 2cm-3cm. The method provided by the invention can be used for rapidly propagating chloranthy florida var. plena, not only can save costs for culturing seedlings and improve production efficiency, but also is not restricted by external conditions, can be carried out in four seasons, saves seedling occupied area, can form a great amount of excellent test-tube seedlings in a short time, and can be used for large-scale and industrialized production.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of clematis double petals. Background technique [0002] Clematis florida Thunb is a vine belonging to the family Ranunculaceae and the genus Clematis. It has high ornamental and medicinal functions, and the root or whole herb is used as medicine for medicinal purposes. Diuretic, regulating qi and laxative, promoting blood circulation and relieving pain. For dysuria, abdominal distension, constipation; external use for joint swelling and pain, insect bites. my country is the origin of clematis, and there are more than 100 varieties, which are born in hilly bushes in low mountainous areas, and most of them are single-petal clematis. Double clematis "Clematis florida var. plena D. Don" is a variety of clematis, with various colors of flowers, 6 petals in the outer circle, and a "ball" composed of finely crushed petals in the inner circle, which has high ornamental value. Clema...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张绪璋叶月琴魏道智汤绍康吴团结陈三辉吴雨英吴枝泉
Owner FUJIAN AGRI & FORESTRY UNIV
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